【摘要】 目的研究雪胆素甲(CuⅡa)对胃癌SGC-7901细胞增殖和迁移能力的影响及其分子作用机制。方法以0、0.01、0.1、1、10、100μmol/L的CuⅡa溶液(A、B、C、D、E、F组)作用SGC-7901细胞48 h后,采用CCK-8法、Transwell小室、流式细胞仪分别检测细胞的增殖能力、迁移能力及细胞凋亡情况。采用免疫印迹(Western blot)法分析细胞中β-连环蛋白(β-catenin)、C-myc、细胞周期素D1(CyclinD1)和糖原合成酶激酶3β(GSK-3β)等蛋白的表达。结果除B组细胞的迁移能力以及凋亡率与A组相比,差异无显著性(P>0.05)外,其他组与A组相比,随着CuⅡa浓度的增加,可以明显地抑制SGC-7901细胞的增殖、迁移及促进SGC-7901细胞的凋亡(F=15.812～98.346,P<0.05),且其作用呈浓度依赖性。与A组相比,0.1、1、10、100μmol/L的CuⅡa作用48 h后,可以降低胃癌细胞β-catenin、C-myc、CyclinD1蛋白的表达(F=15.632、8.494、11.267,P<0.05),增加GSK-3β蛋白的表达(F=21.763,P<0.05),但是B组β-catenin、C-myc、CyclinD1、GSK-3β蛋白表达与A组相比,差异无显著的统计学意义(P>0.05)。结论 CuⅡa能够有效地抑制胃癌细胞的增殖和侵袭,诱导胃癌细胞凋亡,其机制可能与抑制Wnt/β-catenin信号通路的激活有关。更多还原

【Abstract】 Objective To investigate the effect of cucurbitacin Ⅱa(CuⅡa) on the proliferation and migration of gastric cancer SGC-7901 cells and related molecular mechanism. Methods SGC-7901 cells were treated with CuⅡa solution at a concentration of 0, 0.01, 0.1, 1, 10, and 100 μmol/L(groups A, B, C, D, E, and F, respectively) for 48 h, and then the CCK-8 assay, Transwell chamber, and flow cytometry were used to observe the proliferation, migration, and apoptosis of SGC-7901 cells. Wes-tern blot was used to measure the protein expression of β-catenin, C-myc, cyclinD1, and glycogen synthase kinase-3β(GSK-3β) in cells. Results There were no significant differences in the migration ability and apoptotic rate of SGC-7901 cells between groups B and A(P>0.05), while compared with group A, groups C, D, E, and F showed significant inhibition of the proliferation and migration of SGC-7901 cells and significant promotion of the apoptosis of SGC-7901 cells(F=15.812-98.346,P<0.05) in a concentration-dependent manner. Compared with group A, groups C, D, E, and F had significant reductions in the protein expression of β-catenin, C-myc, and cyclinD1(F=15.632,8.494,11.267,P<0.05) and a significant increase in the protein expression of GSK-3β(F=21.763,P<0.05) after 48-hour treatment with CuⅡa solution at a concentration of 0.1, 1, 10, and 100 μmol/L. There were no significant differences in the protein expression of β-catenin, C-myc, cyclinD1, and GSK-3β between groups B and A(P>0.05). Conclusion CuⅡa can effectively inhibit the proliferation and invasion of gastric cancer cells and induce apoptosis of gastric cancer cells, possibly by inhibiting activation of the Wnt/β-catenin signaling pathway.更多还原