【摘要】 目的建立高效液相色谱-荧光检测法测定植物性食品如蔬菜、水果及粮食中多环芳烃含量,了解河北省内上述食品中14种欧盟优控多环芳烃的实际污染状况。方法样品经环己烷-乙酸乙酯(1:1,V:V)溶液超声提取后,浓缩至近干,经正己烷复溶后通过多环芳烃专用固相萃取柱进行净化处理,洗脱液经浓缩后用乙腈定容,采用高效液相色谱-荧光检测法进行检测。结果 14种化合物的线性范围为1.00～18.0μg/kg;方法回收率为60.9%～125.4%,相对标准偏差为2.2%～7.6%;苯并(c)芴、苯并(a)蒽、?、5-甲基-1,2-苯并菲、苯并(j)荧蒽、苯并(b)荧蒽、苯并(k)荧蒽、苯并(a)芘、二苯并(a,e)芘、二苯并(a,i)芘、二苯并(a,h)芘检出限为0.15μg/kg;二苯并(a,l)芘、二苯并(a, h)蒽、苯并(g,h,i)苝、茚并(1,2,3-cd)芘检出限为0.30μg/kg。市售的96份样品中,检出不同种类不同含量的多环芳烃类化合物,检出率为51.0%。结论本方法重现性好,检测灵敏度高,可用于水果、蔬菜及粮食中14种多环芳烃的检测。更多还原

【Abstract】 Objective To establish a method for the determination of polycyclic aromatic hydrocarbons(PAHs) in plant-orgin foods including vegetables, fruits and cereal products by high performance liquid chromatography-fluorescence detector(HPLC-FLD), and understand the actual pollution status of the 14 EU-controlled polycyclic aromatic hydrocarbons in the above-mentioned foods in Hebei province. Methods PAHs were extracted by ultrasonic extraction with cyclohexane-ethyl acetate(1:1, V:V), concentrated to nearly dry, redissolved with n-hexane and then purified by special purification column for PAHs. The eluent was concentrated, and the volume was fixed with acetonitrile. The contents of 14 PAHs were determined by HPLC-FLD. Results The linear range of 14 compounds was 1.00-18.0 μg/kg. The recovery rate was 60.9%-125.4%, and the relative standard deviation was 2.2%-7.6%. The detection limits of target compounds such as benzo(c) fluorene, benzo(a) anthracene, chrysene, 5-methyl chrysene, benzo(j) fluoranthene, benzo(b) fluoranthene, benzo(k) fluoranthene, benzo(a) pyrene, dibenzo(a,e) pyrene, dibenzo(a,i) pyrene and dibenzo(a,h) pyrene were 0.15 μg/kg. The detection limits of dibenzo(a,l) pyrene, dibenzo(a,h) fluoranthene, benzo(g,h,i) perylene, indene(1,2,3-cd) pyrene were 0.30 μg/kg. A total of 96 samples were collected, and the pollution of PAHs were detected in various foods on the market, and the detection rate was 51.0%. Conclusion The method has good reproducibility and high sensitivity, and can be used for the determination of 14 PAHs in vegetables, fruits and cereal products.更多还原