【摘要】 目的建立超高效液相色谱-串联质谱法测定大豆中金雀异黄素(genistein)、大豆苷元(daidzein)和黄豆黄素(glycitein)的含量。方法样品经过乙醇-水(3:1,V:V)提取,用AcquityUPLC?HSST3柱(3.0mm×150mm,1.8μm)分离,以甲醇和0.1%甲酸溶液作为流动相进行梯度洗脱,电喷雾负离子模式(electrospray ionization, ESI-)电离,多反应监测模式(multiple reaction monitoring mode, MRM)进行检测。结果金雀异黄素、大豆苷元和黄豆黄素在2.0～200μg/L范围内线性关系良好(r2>0.999),方法的检出限分别为0.5、0.4、0.1 mg/kg,在2.5、10.0、40.0 mg/kg添加水平的平均回收率为79.3%～107%,相对标准偏差(n=6)为1.3%～6.8%。结论方法简单、灵敏、准确可靠,适用于大豆中金雀异黄素、大豆苷元和黄豆黄素等3种异黄酮素含量的测定。更多还原

【Abstract】 Objective To establish a method for determination of genistein, daidzein, glycitein in soybean by ultra performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS). Method The target in the samples was extracted by ethanol-water(3:1, V:V), separated by Acquity UPLC? HSS T3 column(3.0 mm ×150 mm, 1.8 μm) with methanol and 0.1% formic acid solution as mobile phase for gradient elution, then detected by UPLC-MS/MS under the electrospray ionization(ESI-) anion ion mode with multiple reaction monitoring mode(MRM). Result The results showed that genistein, daidzein and glycitein had good linearity in the range of 2.0– 200 μg/L(r2>0.999), the detection limits of the method were 0.5, 0.4, 0.1 mg/kg, respectively, and the average recovery rates at the addition levels of 2.5, 10.0, and 40.0 mg/kg were 79.3%–107%, while the relative standard deviations(n=6) were 1.3% to 6.8%. Conclusion This method is simple, sensitive and accurate, which is suitable for the determination of isoflavones including genistein, daidzein and glycitein from soybean.更多还原