【摘要】 目的探讨化学发光法和酶联免疫吸附试验(ELISA)检测严重急性呼吸综合征冠状病毒2(SARS-CoV-2)IgG抗体和IgM抗体的性能。方法分别采用化学发光法和ELISA检测116例新型冠状病毒肺炎(COVID-19)患者和346例非COVID-19者的血清SARS-CoV-2 IgG抗体和IgM抗体。结果化学发光法和ELISA检测血清SARS-CoV-2 IgG抗体的敏感性分别为90.52%、63.79%,特异性分别为98.84%、96.53%,准确性分别为96.75%、88.31%;检测血清SARS-CoV-2 IgM抗体的敏感性分别为75.86%和58.62%,特异性分别为99.42%和97.4%,准确性分别96.86%和87.66%。结论化学发光法和ELISA检测血清SARS-CoV-2IgG抗体和IgM抗体的敏感性、特异性、准确性等均较高,临床实验室可根据实际情况选择合适的方法。更多还原

【Abstract】 Objective To determine the immunoglobulin G(IgG) and IgM antibodies to severe acute respiratory syndrome coronavirus 2(SARS-CoV-2) by chemiluminescence and enzyme-linked immunosorbent assay(ELISA). Methods A total of 116 patients diagnosed as COVID-19 and 346 patients without COVID-19 were enrolled,and serum SARS-CoV-2 IgG and IgM antibodies were determined by chemiluminescence and ELISA,respectively. Results The sensitivities,specificities and accuracies of chemiluminescence and ELISA determining serum SARS-CoV-2 IgG were 90.52% and 63.79%,98.84% and 96.53%,96.75% and 88.31%,respectively. The sensitivities,specificities and accuracies of determining serum SARS-CoV-2 IgM were 75.86% and 58.62%,99.42% and 97.4%,96.86% and 87.66%,respectively. Conclusions The determinations of serum SARS-CoV-2 IgG and IgM antibodies by chemiluminescence and ELISA have high sensitivities,specificities and accuracies. Clinical laboratories may choose suitable method according to actual situation.更多还原