【摘要】 来源于特异性干细胞,经体外三维培养,形成与原器官相类似的空间形态结构,称为类器官(organoid)。运用该培养技术形成的肿瘤类器官,可以较好地再现原发肿瘤的体内特征及异质性,而且所需形成时间短、传代稳定,弥补了传统肿瘤模型的缺陷,可用于肿瘤的发生和发展机制研究、药物筛选与个体化治疗、免疫治疗和液体活检等。本文将从肿瘤类器官模型的发展简史、建立方法、应用前景及局限性等方面进行综述。更多还原

【Abstract】 The organoid is derived from specific stem cells for in vitro three-dimensional culture to form a spatial structure, which keeps similar biological characteristics to the original organ. By this culture technology, the tumor organoids can be established. It well retains the feature in vivo and heterogeneity of the original tumor tissues, displays the advantages of short culture cycle and stable genomes after multiple passages, which compensates the defects of traditional tumor models. These tumor organoids can be used to study the mechanism of tumor development, drug screening and individualized treatment, immunotherapy, liquid biopsy, etc. In this paper, the development history,establishment methods, application prospects and limitations of tumor organoid models are reviewed.更多还原