【摘要】 目的·分析原癌基因蛋白FOSB与细胞周期蛋白P16在乳腺浸润性导管癌不同分子亚型组织中的表达差异及相关性。方法·收集2013年8月—2019年3月在中山市博爱医院行手术治疗且术后病理诊断为乳腺浸润性导管癌138例患者的肿瘤组织,以正常乳腺组织作为对照。采用免疫组织化学法分别检测FOSB和P16的表达,分析乳腺癌组织中两者表达水平的相关性,以及它们在Luminal A型、Luminal B型[根据是否表达人表皮生长因子2(human epidermal receptor2,HER2)又分为HER2~-和HER2~+]、HER2阳性型及三阴性乳腺癌(triple-negative breast cancer,TNBC)中的表达情况及相关性。结果·①在138例乳腺浸润性导管癌组织中FOSB与P16的表达水平呈负相关(r=-0.181,P=0.033)。②FOSB在Luminal A型乳腺癌组织中的阳性表达率显著高于其他亚型(P=0.028,P=0.033,P=0.001,P=0.010),而P16在TNBC中的阳性表达率显著高于其他亚型(P=0.025,P=0.005,P=0.008,P=0.011)。③FOSB与P16的表达水平在TNBC组织中呈负相关(r=-0.566,P=0.018)。结论·FOSB与P16在乳腺浸润性导管癌不同分子亚型组织中的表达存在差异,FOSB在Luminal A型中表达水平较高,P16在TNBC中表达水平较高;两者在TNBC中的表达呈负相关。更多还原

【Abstract】 Objective · To detect the expressions of proto-oncogene protein FOSB and cyclin protein P16 in the different molecular subtypes of breast invasive ductal cancer and their correlation.Methods · The tumor tissues of 138 patients diagnosed with breast invasive ductal cancer by postoperative pathological examinations in Zhongshan Bo’ai Hospital from August 2013 to March 2019 were collected with normal breast tissues as control.Immunohistochemical staining was performed for the expressions of FOSB and P16 to investigate their correlation in the breast cancer.Besides,their expressions and their correlations in the different molecular subtypes of breast cancers,i.e.Luminal A,Luminal B[According to the expression of human epidermal growth factor 2 (HER2),it can be divided into HER2~-and HER2~+.],HER2 positive subtype,and triple-negative breast cancer (TNBC),were also detected.Results · ① The expression level of FOSB in 138 cases of breast invasive ductal cancer tissues was negatively correlated with P16 (r=-0.181,P=0.033).② The expression of FOSB was significantly higher in Luminal A than those in other subtypes (P=0.028,P=0.033,P=0.001,P=0.010),while the expression of P16 was significantly higher in TBNC than those in other subtypes (P=0.025,P=0.005,P=0.008,P=0.011).③ The expression level of FOSB in TBNC was negatively correlated with the expression of P16 (r=-0.566,P=0.018).Conclusion · The expressions of FOSB and P16 in the different molecular subtypes of breast invasive ductal cancer are different.The expression level of FOSB significantly increases in Luminal A,and that of P16 significantly increases in TBNC.There is a negative correlation between the expression levels of FOSB and P16 in TBNC.更多还原