【摘要】 目的分析RecQ5在不同组织和细胞上的表达差异,推断RecQ5与组织恶性程度间的关系。方法选取骨肉瘤标本共35例、癌旁组织标本20例及正常骨组织标本20例,通过免疫组织化学染色法检测不同组织中RecQ5的表达情况,并进一步分析其表达程度与骨肉瘤分期的关系;培养hFOB1.19、U2OS和MG-63细胞株,通过Real-timePCR和Westernblot法检测RecQ5的表达程度。结果 RecQ5在人骨肉瘤组织中低表达。正常骨组织中,RecQ5的表达阳性率为100%(20/20),在癌旁组织中的表达阳性率为90%(18/20),而在骨肉瘤组织中,RecQ5表达阳性率为68.5%(24/35);正常骨组织、癌旁组织与骨肉瘤组织的RecQ5表达阳性率之间比较差异具有统计学意义(P=0.004),但正常骨组织与癌旁组织之间的阳性率差异无统计学意义(P>0.05)。RecQ5的表达强度随着组织恶性程度的不断增加而逐渐减弱,差异具有统计学意义(P<0.05),且在骨肉瘤组织中,其也随着肿瘤分期增加而表达强度逐渐减弱(P<0.05)。RecQ5在人骨肉瘤细胞株MG-63和U2OS中mRNA和蛋白表达水平较正常人成骨细胞hFOB1.19中低,差异有统计学意义(P<0.05)。结论 RecQ5的表达水平与组织的恶性程度呈负相关,RecQ5的缺失与骨肉瘤的发生存在相关性。更多还原

【Abstract】 Objective To analyze the different expression of RecQ5 in different tissues and cells,inferring the relationship between RecQ5 expression and the degree of malignancy in different tissues. Methods 35 OS samples,20 tumor adjacent tissues samples and 20 normal bone samples were chosen for the research.We compared the expression of RecQ5 among OS tissues,tumor adjacenttissues and bone tissues by immunohistochemical method and analyzed the relationship between expression intensity of RecQ5 and malignant degree of tissues,including AJCC staging.Moreover,Real-time PCR and Western blot were used to detect the mRNA and protein level of RecQ5 in hFOB1.19,U2 OS and MG-63 cell lines. Results RecQ5 expression decreased inhuman osteosarcoma tissue.According to immunohistochemical results,the positive rate of RecQ5 expression was 100%(20/20)in normal bone tissues and positive rate was 90%(18/20) in tumor adjacent tissues,while the positive rate was 68.5%(24/35)in osteosarcoma tissues,which had statistically significant difference between the former two(P=0.004),but no significant difference in the former two(P>0.05).Further analysis of relationship between expression intensity and malignancy degree of tissues found that the expression intensity of RecQ5 decreased with increasing of malignant degree of tissues(P<0.05)and OS staging(P<0.005),which showed the expression of RecQ5 was negatively correlated with malignancy degree of tissues.Both Real-time PCR and Western blot showed the mRNA and protein level of RecQ5 expression was reduced in MG-63 and U2 OS cells,compared to hFOB 1.19 cells(P<0.05). Conclusion The expression level of RecQ5 is negatively correlated with the malignancy degree of tissues.Deletion of RecQ5 is associated with the occurrence of osteosarcoma.更多还原