【摘要】 目的探讨胰腺癌组织中微小RNA-939-5p(miR-939-5p)与Rho GTPase激活蛋白4(ARHGAP4)的表达变化及临床意义。方法用荧光定量PCR技术检测98例胰腺癌组织及癌旁正常组织中miR-939-5p、ARHGAP4mRNA的相对表达量,并分析二者表达与胰腺癌患者临床病理特征的关系,用Pearson线性相关法分析miR-939-5p、ARHGAP4 mRNA的关系。结果与癌旁正常组织比较,胰腺癌组织中miR-939-5p相对表达量高,ARHGAP4mRNA相对表达量低(P均<0.001)。胰腺癌组织中miR-939-5p、ARHGAP4 mRNA表达与肿瘤TNM分期、组织分化程度、淋巴结转移有关(P均<0.05),与患者年龄、性别及肿瘤直径无关(P均> 0.05)。胰腺癌组织中miR-939-5p表达与ARHGAP4 mRNA表达呈负相关(r=-0.574,P<0.05)。结论胰腺癌组织中miR-939-5p呈高表达,ARHGAP4 mRNA呈低表达,二者可能协同参与肿瘤的发生发展。更多还原

【Abstract】 Objective To investigate the expression changes and clinical significance of microRNA-999-5 p( miR-999-5 p) and Rho GTPase activating protein 4(ARHGAP4) in the pancreatic cancer tissues.Methods Quantitative real-time PCR(qRT-PCR) was used to detect the relative expression of miR-939-5 p and ARHGAP4 mRNA in 98 cases of pancreatic cancer tissues and adjacent normal tissues, and the relationship of the two indicators with clinicopathological characteristics was analyzed.Pearson linear correlation method was used to analyze the relationship between miR-939-5 p and ARHGAP4 mRNA.Results Compared with the adjacent normal tissues, the relative expression of miR-939-5 p in the pancreatic cancer tissues was higher, and the relative expression of ARHGAP4 mRNA was lower(allP< 0.001).The expression levels of miR-939-5 p and ARHGAP4 mRNA in the pancreatic cancer tissues were related to tumor TNM stage, cell differentiation, and lymph node metastasis(allP< 0.05), but were not related to age, gender or tumor diameter(allP>0.05).The expression of miR-939-5 p in the pancreatic cancer tissues was negatively correlated with the expression of ARHGAP4 mRNA(r=-0.574,P< 0.05).Conclusion In the pancreatic cancer tissues, miR-939-5 p is highly expressed, and ARHGAP4 mRNA is low expressed, and both of them may be synergistically involved in the occurrence and development of tumors.更多还原