【摘要】 目的观察不同浓度七氟烷对人骨肉瘤saos2细胞增殖和迁移能力的影响,并探讨其作用机制。方法体外培养人骨肉瘤saos2细胞,随机分为1. 7%七氟烷组、3. 4%七氟烷组及5. 1%七氟烷组,分别暴露于1. 7%、3. 4%、5. 1%的七氟烷中2 h,继续培养24 h。另设对照组,正常培养,不进行七氟烷暴露处理。采用CCK-8法检测各组细胞增殖能力,细胞划痕实验观察细胞迁移能力,Western blotting法检测细胞上皮间质转化相关蛋白E型钙黏蛋白（E-cadherin）、波形蛋白（Vimentin）及丝裂原活化的蛋白激酶（MAPK）信号通路相关蛋白转化生长因子β₁（TGF-β₁）、基质金属蛋白酶2（MMP-2）表达。结果不同浓度七氟烷组细胞增殖抑制率均高于对照组,5. 1%七氟烷组增殖抑制率高于1. 7%、3. 4%七氟烷组（P均<0. 05）。不同浓度七氟烷组划痕愈合率均低于对照组,5. 1%七氟烷组划痕愈合率低于1. 7%、3. 4%七氟烷组（P均<0. 05）。不同浓度七氟烷组E-cadherin蛋白表达均高于对照组,Vimentin蛋白、TGF-β₁、MMP-2蛋白表达均低于对照组（P均<0. 05）,其中5. 1%七氟烷组蛋白表达变化大于1. 7%、3. 4%七氟烷组（P均<0. 05）。结论 1. 7%、3. 4%及5. 1%七氟烷均能够抑制人骨肉瘤saos2细胞的增殖和迁移能力,其中以5. 1%七氟烷作用最强;其机制可能与抑制上皮间质转化以及下调MAPK信号通路活性有关。更多还原

【Abstract】 Objective To observe the effects of sevoflurane at different concentrations on the proliferation and migration of human osteosarcoma saos2 cells. Methods Human osteosarcoma saos2 cells were cultured in vitro and randomly divided into 1. 7% sevoflurane group,3. 4% sevoflurane group,and 5. 1% sevoflurane group which were exposed to1. 7%,3. 4% and 5. 1% sevoflurane for 2 h,and then were cultured for 24 h. A control group was set up without sevoflurane exposure. CCK-8 method was used to evaluate the cell proliferation ability,and the scratch test was used to determine the effect of sevoflurane on the migration ability of osteosarcoma saos2 cells. The expression levels of epithelial-mesenchymal transition-related proteins E-cadherin,Vimentin,and mitogen-activated protein kinase（MAPK） pathway-related proteins transforming growth factor β₁（TGF-β₁） and matrix metalloproteinase 2（MMP-2） were detected by Western blotting.Results The inhibition rates of cell proliferation in different concentrations of sevoflurane groups were higher than that in the control group,the inhibition rate of proliferation in the 5. 1% sevoflurane group was higher than those in the 1. 7%,3. 4% sevoflurane groups（all P < 0. 05）. The scratch healing rates in the sevoflurane groups with different concentrations were lower than that in the control group,and the scratch healing rate in the 5. 1% sevoflurane group was lower than those in the 1. 7% and 3. 4% sevoflurane groups（all P < 0. 05）. The sevoflurane groups with different concentrations had higher expression levels of E-cadherin protein than the control group,and had lower expression of Vimentin protein,TGF-β₁,and MMP-2 protein than the control group（all P < 0. 05）; the protein change of the 5. 1% sevoflurane group was greater than those of the 1. 7% and 3. 4% sevoflurane groups（all P < 0. 05）. Conclusion 1. 7%,3. 4% and 5. 1% sevoflurane can inhibit the proliferation and migration abilities of human osteosarcoma saos2 cells,and the mechanism may be related to the inhibition of epithelial-mesenchymal transition and down-regulation of MAPK signaling pathway activity.更多还原