【摘要】 目的本研究建立了一种直接检测化妆品中氟喹诺酮类抗生素（FQs）的高特异性化学发光酶联免疫吸附试验（CL-ELISA）方法。方法辣根过氧化物酶（HRP）经高碘酸钠活化与抗氧氟沙星抗体进行偶联反应后,加入NaBH₄饱和甲醇溶液,充分进行还原反应后得到辣根过氧化物酶-氧氟沙星抗体标记物,采用直接竞争化学发光免疫法对抗体标记物进行检测。结果氟喹诺酮类检测限LOD(IC₁₀)为2.0 ng·mL^-1;检测范围(IC₂₀～IC₈₀)为4.1～104.6 ng·mL^-1;平均回收率范围在75.2%～98.2%,变异系数低于9.64%。交叉反应率结果表明该方法对恩诺沙星、环丙沙星、诺氟沙星、培氟沙星、洛美沙星、沙拉沙星等氟喹诺酮类抗生素有不同程度的交叉反应（12.5%～128.6%）。结论该方法简单、快速、准确度高,可用于化妆品中多种氟喹诺酮类抗生素高灵敏度快速检测。更多还原

【Abstract】 Objective This research presented a direct high-specific chemiluminescent enzyme-linked immunosorbent assay protocol（CL-ELISA） protocol for monitoring ofloxacin in cosmetics.Methods Horseradish peroxidase HRP was activated by sodium periodate and coupled with anti-ofloxacin antibody.After adding NaBH₄ saturated methanol solution,the HRP-ofloxacin antibody marker was obtained by full reduction reaction.The antibody marker was detected by direct competitive chemiluminescence immunoassay.Results The limit of detection（LOD） value,measured by IC₁₀,was 2.0 ng mL^-1.The detection range,measured by IC₂₀～IC₈₀,was 4.1～104.6 ng mL^-1.In spiked cosmetics samples,mean recoveries ranged from 75.2% to 98.2%,with variation less than 9.64%.This developed protocol showed different cross-reactivity value for fluoroquinolones tested（12.5%～128.6%）.Conclusion The method was simple,rapid and accurate,and can be used for the rapid detection of fluoroquinolones in cosmetics with high specificity.更多还原