【摘要】 目的建立以2,4-二硝基氟苯为衍生化试剂,高效液相色谱法（HPLC）测定复方氨基酸注射液（18AA）中各氨基酸含量的方法。方法采用Agilent HC-C₁₈色谱柱,乙腈-水（60∶40）为流动相A,乙腈-磷酸盐缓冲液（9∶91）流动相B,梯度洗脱,流速1.0 m L·min^-1,检测波长360 nm,柱温27℃。结果复方氨基酸注射液中各氨基酸分离度良好,线性相关系数均大于0.998 9,平均回收率均在98%～102%之间,RSD%均小于2%。结论本方法可以用于复方氨基酸注射液（18AA）中氨基酸检测。更多还原

【Abstract】 Objective To establish a method for the determination of amino acids in compound amino acid injection（18 AA） by high performance liquid chromatography（HPLC） with 2,4-dinitrofluorobenzene as derivative reagent.Methods An Agilent HC-C18 column was used in the gradient elution with acetonitrile-water（60 ∶ 40） as mobile phase A and acetonitrile-phosphate buffer（9 ∶ 91） as mobile phase B.The flow rate was 1.0 m L·min^-1,the detection wavelength was 360 nm and the column temperature was 27 ℃ .Results The separation degree of amino acids in compound amino acid injection was good.All the linear correlation coefficients were greater than 0.998 9.The average recoveries were between 98%and 102% with RSDs were less than 2%.Conclusion This method can be used for the determination of amino acids in compound amino acid injection（18 AA）.更多还原