【摘要】 建立了固相萃取-高效液相色谱同时测定果蔬中吡虫啉和多菌灵含量的方法。试样用乙腈提取,经Cleanert-NH2固相萃取小柱净化,流动相定容后,采用高效液相色谱法测定。以甲醇-水(32∶68)为流动相,流速1.0 m L/min,于270 nm波长下检测。结果表明,吡虫啉和多菌灵分别在0.02μg/m L～1.0μg/m L和0.05μg/m L～5.0μg/m L范围内线性良好,相关系数均0.999 9以上;回收率在85.1%～100.5%之间,相对标准偏差为1.6%～6.6%(n=6)。该方法简便、高效,适用于果蔬中吡虫啉和多菌灵含量的同时测定。更多还原

【Abstract】 A method for the simultaneous determination of imidacloprid and carbendazim in fruits and vegetables was developed by solid-phase extraction and high performance liquid chromatography(SPE-HPLC). The samples was extracted with acetonitrile and purified by a Cleanert-NH2 solid-phase extraction(SPE) cartridge. The extract was analyzed by HPLC with methanol-water(32∶68) as the mobile phase. The flow rate of the mobile phase was 1.0 m L/min and the detection wavelength was 270 nm. The results showed that imidacloprid and carbendazim had good linearity in the concentration ranges of 0.02 μg/m L ～1.0 μg/m L and 0.05 μg/m L ～5.0 μg/m L respectively, the correlation coefficient was above 0.9999, the recovery was 85.1% ～100.5%, and the relative standard deviation was1.6%～6.6%(n=6). The method is simple, efficient and suitable for the simultaneous determination of imidacloprid and carbendazim in fruits and vegetables.更多还原