【摘要】 目的采用超高效液相色谱（UPLC）波长切换法同时测定桑叶中新绿原酸、隐绿原酸、绿原酸、芦丁、异槲皮苷、紫云英苷的含量。方法以Waters BEH shield RP₁₈为色谱柱（100 mm×2.1 mm,1.7μm）,以乙腈（A）-0.1%磷酸（B）为流动相,梯度洗脱;柱温40℃,全波长扫描（190～400 nm）,波长切换（0～3min,325 nm,检测新绿原酸、隐绿原酸、绿原酸;3～8 min,358 nm,检测芦丁、异槲皮苷、紫云英苷）。结果桑叶中新绿原酸、隐绿原酸、绿原酸、芦丁、异槲皮苷、紫云英苷6种活性成分在8 min内分离效果理想,质量浓度分别在17.24～33.91μg/ml（r=0.999）,23.01～45.68μg/ml（r=0.999）,50.93～121.93μg/ml（r=0.999）,11.91～54.11μg/ml（r=0.999）,13.99～60.00μg/ml（r=0.999）,4.83～24.67μg/ml（r=0.999）范围内与峰面积呈良好的线性关系;平均加样回收率（n=6）为99.28%～103.75%,RSD≤3.3%,供试品溶液在室温条件下24 h内稳定,RSD<2.4%。11个不同产地桑叶中新绿原酸、隐绿原酸、绿原酸、芦丁、异槲皮苷、紫云英苷6个成分的含量分别为0.86～1.7,1.15～2.28,2.55～6.10,0.60～2.71,0.70～3.00,6.1～16.97 mg/g。结论该方法操作简单,结果准确,专属性强,可为桑叶药材的质量控制标准提高提供参考。更多还原

【Abstract】 Objective To develop a method for simultaneous determination of nochlorogenic acid, cryptochlorogenic acid, chlorogenic acid, rutin, isoquercetin and astragalin in Mori folium by UPLC with wavelength switching. Methods The chromatographic column was Waters BEH shield RP₁₈（100 mm×2.1 mm, 1.7 μm）. Gradient elution was performed, using acetonitrile（A）-0.1 % phosphoric acid（B） as mobile phase. The flow rate was 0.3 ml/min, the column temperature was 40 ℃. The detection wavelength was set at 325 nm for neochlorogenic acid, cryptochlorogenic acid and chlorogenic acid in the first 3 min, and 358 nm for rutin, isoquercetin and astragalin during 3-8 min after full wavelength scanning（190-400 nm）. Results The good saperation of six components was achieved within 8 min. Good linearity was obtained for neochlorogenic acid, cryptochlorogenic acid, chlorogenic acid, rutin, isoquercetin and astragalin in the ranges of 17.24-33.91 μg/ml（r=0.999）, 23.01-45.68 μg/ml（r=0.999）, 50.93-121.93 μg/ml（r=0.999）, 11.91-54.11 μg/ml（r=0.999）, 13.99-60.00 μg/ml（r=0.999） and 4.83-24.67 μg/ml（r=0.999）, respectively. The average recoveries（n=6） were 99.28 %-103.75 %, with RSDs not more than 3.3 %. The six analytes were stable at room temperature within 24 h, with RSDs less than 2.4 %. The contents of six index components in Mori folium from 11 different origins were in the ranges of 0.86-1.7 mg/g for nochlorogenic acid, 1.15-2.28 mg/g for cryptochlorogenic acid, 2.55-6.10 mg/g for chlorogenic acid, 0.60-2.71 mg/g for rutin, 0.70-3.00 mg/g for isoquercetin and 6.1-16.97 mg/g for astragalin. Conclusion The proposed method is simple, accurate and repeatable, which can provide reference for the improvement of the quality control standard of Mori folium.更多还原