【摘要】 目的:研究不同亚型宫颈癌细胞株中葡萄糖调节蛋白78(glucose regulated protein 78,GRP78)表达情况,以及GRP78表达水平对宫颈癌细胞顺铂敏感性的影响。方法:采用免疫组织化学法检测人乳头瘤病毒(human papillomavirus,HPV) 16(+)SiHa、HPV18(+)HeLa以及HPV(-)C33a宫颈癌细胞中的GRP78表达,MMT、Western blot法检测以上3种细胞在不同浓度顺铂、衣霉素+顺铂处理后的细胞抑制率及GRP78表达水平,采用单因素方差分析,分析相同浓度下单用顺铂、衣霉素+顺铂联合处理后3种细胞的细胞抑制率及GRP78蛋白表达的差异。结果:SiHa、HeLa细胞GRP78阳性表达水平明显高于C33a细胞,SiHa细胞GRP78阳性表达水平最高(P<0.01)。随着顺铂浓度增加,3种宫颈癌细胞抑制率增加呈浓度依赖性,且HeLa细胞抑制率最高(P<0.05)。与单独顺铂组相比,衣霉素联合顺铂药物处理组中HeLa及SiHa细胞抑制率显著升高(P<0.05),并且GRP78表达水平也明显升高(P<0.01),且各细胞株中GRP78表达水平均随顺铂浓度增加而增加。结论:GRP78表达水平与宫颈癌细胞亚型有关,SiHa、HeLa细胞对顺铂敏感性高于C33a细胞。衣霉素导致GRP78明显高表达,增加了SiHa、HeLa细胞对顺铂的敏感性。更多还原

【Abstract】 Objective: To study the expression of glucose-regulated protein 78(GRP78) in different subtypes of cervical cancer cells and the effect of GRP78 expression on the sensitivity of cervical cancer cells to cisplatin. Methods: Immunohistochemistry was used to detect the expression of three cervical cancer cells, GRP78 in human papillomavirus(HPV)16-positive SiHa, HPV18 positive-HeLa and HPV-negtive C33 a. Methyl thiazolyl tetrazolium and Western blot were used to detect the inhibition rate and GRP78 level of the above cells treated with different concentration of cisplatin and tunicamycin plus cisplatin. One-way ANOVA was used to analyze the differences in inhibition rate and GRP78 level in three kinds of cells treated with cisplatin and tunicamycin + cisplatin at the same concentration. Results: The GRP78 level in SiHa and HeLa were significantly higher than that in C33 a, and the expression of GRP78 in HeLa was the highest(P<0.01). As the concentration of Cisplatin increased, the inhibition rate increased in a concentration-dependent manner, and the inhibition rate of HeLa was the highest(P<0.05). Compared with the Cisplatin group, the inhibition rate of HeLa and SiHa and the GRP78 level in the tunicamycin plus cisplatin group were significantly higher, and the GRP78 level in all cell lines increased as the concentration of cisplatin increased. Concusion: The GRP78 level is related to the subtypes of cervical cancer cells. The sensitivity of SiHa and HeLa to cisplatin is higher than that of C33 a. Tunicamycin induces higher expression of GRP78, which increases the sensitivity of SiHa and HeLa to cisplatin.更多还原