【摘要】 目的:基于气相色谱-质谱法(GC-MS)技术探讨八角枫对大鼠血浆与尿液代谢的影响。方法:将32只SD大鼠分为对照组及八角枫低、中、高剂量组,每组8只。对照组给予生理盐水灌胃,八角枫低、中、高剂量组分别按0.25、0.50、1.00 g/(kg·d)的剂量灌胃八角枫煎煮液,共14 d。第15天收集各组大鼠血浆与尿液样本,衍生化处理后,进行GC-MS分析。结果:各组间偏最小二乘法判别分析(PLS-DA)评分分离效果不佳,各组间互相有交叉,但血浆的分离效果要好于尿液样本;八角枫各剂量组与对照组偏最小二乘回归3D模型(PLS-3D)评分可以区分;且PLS-3D评分结果较PLS-DA评分结果好。与对照组比较,八角枫高剂量组大鼠血浆中尿素含量升高(P<0.05),尿液戊二酸降低(P<0.05);八角枫中、高剂量组大鼠葡萄糖、黄胺酸和琥珀酰胺酸含量降低(P<0.05);八角枫低剂量组尿液中木糖醇升高(P<0.05)。结论:基于GC-MS技术的代谢组学研究发现,八角枫可诱导大鼠血浆与尿液代谢紊乱,可为其临床安全用药提供毒理、药理等方面的参考。更多还原

【Abstract】 Objective:To study the effect of Radix Alangii Chinensis on metabolism of plasma and urine in rats based on gas chromatography-mass spectrometry(GC-MS) technology. Methods:A total of 32 SD rats were divided into the control group and the Radix Alangii Chinensis groups with low,medium and high dose respectively,8 rats in each group. The control group was given normal saline by gavage,and the Radix Alangii Chinensis groups with low,medium and high dose were given Radix Alangii Chinensis decocted fluid by gavage at the doses of 0.25, 0.50 and 1.00 g/(kg · d) respectively. All the groups were treated for 14 days. On the 15 thday,samples of plasma and urine in each group were collected for derivation,and then GC-MS analysis was performed. Results:The separation effect of partial least squares regression analysis(PLS-DA)scores among the groups was not good,and there was intersection among the groups,but the separation effect of plasma was better than that of urine samples. The partial least squares regression 3 D model(PLS-3 D) scores in the Radix Alangii Chinensis groups with low,medium and high dose respectively could be distinguished from that in the control group;the PLS-3 D scores were better than PLS-DA scores. Compared with those in the control group respectively,the urea content in plasma in the Radix Alangii Chinensis group with high dose was increased(P<0.05),and the content of urinary glutaric acid was decreased(P<0.05);the contents of glucose,flavianic acid and succinamic acid in the Radix Alangii Chinensis groups with medium and high dose respectively were decreased(P<0.05); the content of xylitol in urine in the Radix Alangii Chinensis group with low dose was increased(P < 0.05). Conclusion : The study of metabonomics based on GC-MS technology finds that Radix Alangii Chinensis can induce the metabolic disorder of plasma and urine in rats, and provide references on toxicology and pharmacology for clinical safe medication.更多还原