【摘要】 本文研究了0.25～3 mmol/L浓度的NaHSO₃对紫球藻（Porphyridium violaceum）和0.06～0.96 mmol/L浓度的NaHSO₃对蓝隐藻（Chroomonas placoidea）的叶绿素荧光参数（PSII最大光能转化效率F_v/F_m、相对电子传递速率rETR、光化学淬灭qP、非光化学淬灭NPQ）、细胞密度、干重、胞外多糖和藻胆蛋白含量的影响。研究表明,较低浓度的NaHSO₃对紫球藻和蓝隐藻的生长及活性物质积累有促进作用,2种微藻进行生长和活性物质积累的最适NaHSO₃浓度分别为0.5和0.12 mmol/L,此条件下,实验结束时,2种微藻的F_v/F_m、rETR、qP、细胞密度、干重、胞外多糖和藻胆蛋白含量均达到最大值,显著高于对照组。其中,紫球藻的最终细胞密度、干重、胞外多糖和藻胆蛋白含量分别比对照组增加了36.0%、19.1%、71.8%和69.0%。蓝隐藻的上述参数在NaHSO₃浓度为0.12 mmol/L时则比对照组增加了60.8%、45.4%、60.0%和53.1%。另一方面,NaHSO₃浓度为2～3 mmol/L时显著抑制紫球藻生长及活性物质积累,NaHSO₃浓度为0.48～0.96 mmol/L则不利于蓝隐藻生长及活性物质积累。研究结果表明,适合紫球藻和蓝隐藻生长及胞外多糖和藻胆蛋白积累的最佳NaHSO₃浓度分别为0.5和0.12 mmol/L,该研究为2种微藻的开发利用提供了理论依据。更多还原

【Abstract】 Effects of NaHSO₃ at 0.25～3 and 0.06～0.96 mmol/L on the maximal photochemical efficiency of PSII（F_v/F_m）, relative electron transport rate（rETR）, photochemical quenching（qP）, non-photochemical quenching（NPQ）, cell density, dry weight, extracellular polysaccharide and phycobiliprotein contents of Porphyridium violaceum and Chroomonas placoidea were investigated, respectively. The results showed that lower concentrations of NaHSO₃ had positive effects on the growth and bioactive substances accumulation of two microalgae.The optimal NaHSO₃ concentration for the growth and bioactive substances accumulation of P. violaceum and C. placoidea was 0.5 and 0.12 mmol/L, respectively, at which, F_v/F_m, rETR, qP, cell density, dry weight, extracellular polysaccharide and phycobiliprotein contents of two microalgae all reached the maximum levels at the end of experiment,which were significantly higher than those of the control.Compared with the control, the cell density, dry weight, extracellular polysaccharide and phycobiliprotein contents of P. violaceum at 0.5 mmol/L increased by 36.0%, 19.1%, 71.8% and 69.0%, respectively, while those of C. placoidea at 0.12 mmol/L increased by 60.8%, 45.4%, 60.0% and 53.1%, respectively. On the other hand, 2～3 mmol/L of NaHSO₃ inhibited the growth and bioactive substances accumulation of P. violaceum observably while 0.48～0.96 mmol/L of NaHSO₃was not conducive to the growth and bioactive substances accumulation of C. placoidea. The results indicated that the optimal NaHSO₃ concentration for the growth and extracellular polysaccharide and phycobiliprotein accumulation of P. violaceum and C. placoidea was 0.5 and 0.12 mmol/L, respectively,which should aid to the exploration of these two microalgae.更多还原