【摘要】 病原菌分泌的多肽可作为植物防御反应的激发因子。大丽轮枝孢(Verticillium dahliae) Asp f2类蛋白(VDAL)为大丽轮枝孢(俗称黄萎菌)的外泌蛋白,属于烟曲霉(Aspergillus fumigatus) f2类锌离子结合蛋白。通过工程菌发酵等工艺将棉花(Gossypium spp.)黄萎菌Vd146菌系VDAL146蛋白制成干粉,其稀释液喷施于叶面,能够激活水稻(Oryza sativa)等植物的免疫反应,并促进植物生长发育。本研究对VDAL146 (MT974503)进行分离和鉴定,在水稻破口期前后两次叶面喷施VDAL146。结果显示,水稻对纹枯病的抗性显著提高,穗粒数和千粒重增加,最终增产14.5%;同时,VDAL146没有显示直接的抑菌活性。另外,转录组分析证明,VDAL146处理使322个基因上调表达,主要涉及碳代谢、有机磷与葡聚糖生物合成以及ATP结合等信号通路。综上所述,VDAL146在生物刺激剂方面具有开发潜力,可增强水稻抗病性和促进水稻生长。更多还原

【Abstract】 Peptides secreted by pathogens serve as elicitors of plant defense responses. Verticillium dahliae Aspf2-like protein(VDAL) is secretory protein of V. dahliae and belongs to Aspergillus fumigatus f2-like zincbinding protein. The VDAL146 protein in V. dahliae Vd146 strain of cotton(Gossypium spp.) was made into dry powder by engineering bacteria fermentation, and its diluent was sprayed on the leaves, which could activate the immune response of plants such as rice(Oryza sativa) and promote the growth and development of plants. In present study, VDAL146(MT974503) was isolated and characterized, and sprayed twice before and after rice break stage. The results showed enhanced resistance to rice sheath blight, as well as improved yield by 14.5% via increasing both the number of grains per panicle and thousand grain weight. At the same time,VDAL146 did not show direct fungicidal activity. In addition, transcriptome analysis indicated that VDAL146 treatment resulted in upregulation of 322 genes involved in carbohydrate metabolism, organophosphate and glucan biosynthesis, and ATP binding. In conclusion, VDAL146 holds the potential to be developed in fungicide and biostimulant to enhance disease resistance and growth promotion in rice crop.更多还原