【摘要】 长期以来,植物线虫的鉴定和分类主要以形态学特征和测量数据为依据,有关的方法主观且繁琐,难以可靠地鉴别线虫种内和种间群体间差异,而以DNA为基础的分子分类学研究方法,提供的信息客观简便,更能揭示不同类群的线虫在进化过程中的系统学关系。本研究分别利用NEMF1/S3和A/S3两对引物对来源于国内外植物根围土壤的小杆目、矛线目和三矛目94个植物寄生线虫种群的18S rRNA基因进行PCR扩增,所得序列经DNAstar软件联配比较、编辑,并用MEGA 6.0软件进行系统发育分析,以最大似然法(maximum likelihood, ML)构建系统发育树,结果发现,基于引物对A/S3扩增序列构建的植物线虫系统发育树能系统体现出不同线虫类群及同一线虫类群内不同种群间的系统发育关系。相关研究结果将为未来植物线虫系统进化研究提供一定的借鉴。更多还原

【Abstract】 Traditional methods for identification and classification of plant nematodes are mainly based on morphological characters and morphometrics. These methods are subjective and cumbersome, and are difficult to make reliably taxonomy and identification. The molecular method for taxonomy and identification based on DNA not only provides objective information, but also can reveal the evolution of different groups of nematodes. In this study, 18 S r RNA gene fragments of 94 plant parasitic nematode populations, which belonging to Rhabditida, Dorylaimida and Triplonchida, were amplified with primer sets NEMF1/S3 and A/S3, respectively. The sequences were compared and edited by DNAstar software, and were used to construct the phylogenetic trees by Mega 6.0 software. The results showed that the phylogenetic tree constructed based on the sequences, which PCR amplified with primers A/S3, can reflect the phylogenetic relations of nematode species within different groups and the same group. The results provide reference to study phylogeny of plant nematodes in the future.更多还原