【摘要】 为研究基于代谢组学的藜麦花期在水分胁迫条件下的响应机制,设置了干旱胁迫和水涝胁迫2种处理,研究了处理后藜麦的生理指标变化和代谢物质差异。结果表明,干旱胁迫和水涝胁迫处理后,生理指标变化趋势基本一致,SP含量下降,WSS含量、POD活性和T-AOC显著增加,干旱胁迫组的MDA含量显著增加,WW组的MDA增加不显著。代谢物质变化显著,干旱胁迫组中共鉴定出60种差异代谢物,7种上调,53种下调,主要包括16种氨基酸类、16种糖类及衍生物、7种有机酸类和5种脂肪酸类,共注释到46条代谢通路;水涝胁迫组中共鉴定出74种差异代谢物,57种上调,17种下调,主要包括16种氨基酸类、19种糖类及衍生物、14种有机酸类和5种脂肪酸类,共注释到47条代谢通路。藜麦花期对干旱和水涝胁迫的响应途径基本一致,主要依靠小分子渗透调节物质的积累及氨基酸、糖和有机酸的相关代谢途径来响应水分胁迫。更多还原

【Abstract】 In order to study the response mechanism of quinoa at flowering stage based on metabolomics under water stress,two stress treatments,drought Chenopodium and waterlogging,were set up,and the physiological index changes and metabolite differences of quinoa after treatment were studied.The results showed that after drought and waterlogging stress treaments,the physiological index change trends were basically the same,soluble protein(SP) content decreased,water soluble sugars(WSS) content,peroxidase(POD) activity and total antioxidant capacity(T-AOC) increased significantly,malondialdehyde(MDA) content increased significantly in drought stress group,and increased insignificantly in waterlogging stress group.The metabolites changed significantly.A total of 60 differential metabolites were identified in the drought stress group,7 were up-regulated,53 were down-regulated,mainly including 16 amino acids,16 sugars and derivatives,7 organic acids and 5 fatty acids,and 46 metabolic pathways were annotated; In the waterlogging stress group,there were 74 differential metabolites identified,57 were up-regulated,17 were down-regulated,mainly including 16 amino acids,19 sugars and derivatives,14 organic acids and 5 fatty acids,and a total of 47 metabolic pathways are annotated.The response pathway of quinoa to drought and waterlogging stress at flowering stage was basically the same,which mainly depended on the accumulation of small molecular osmotic regulators and the related metabolic pathways of amino acids,sugars and organic acids to respond to water stress.更多还原