【摘要】 以菠萝（Ananas comosus （Linn.） Merr.）果实为材料,通过RT-PCR分别扩增液泡膜水通道蛋白基因（AcTIP1-1）（GenBank登录号:XM₀20238793.1）的开放阅读框（ORF）和编码区（CDS）序列,序列长度分别为753 bp和750 bp.将ORF序列亚克隆到载体pET32a（+）上,CDS序列被亚克隆到载体pSP64 Poly（A）上,构建成重组载体pET32a（+）-AcTIP1-1和pSP64 Poly（A）-AcTIP1-1.pET32a（+）-AcTIP1-1可为诱导表达并纯化融合蛋白制备抗体,pSP64 Poly（A）-AcTIP1-1可为制备含有Poly（A）+的AcTIP1-1转录子奠定基础.更多还原

【Abstract】 The open reading frame（ORF）sequence and coding sequence（CDS）of tonoplast intrinsic protein gene（AcTIP1-1）from pineapple（Ananas comosus（Linn.）Merr.）GenBank（Accession number:XM₀20238793.1）was amplified by RT-PCR. The sequence lengths of ORF and CDS were 753 bp and 750 bp. The ORF sequence was sub-cloned into the prokaryotic expression vector p ET32 a（+）and the CDS sequence was sub-cloned into the vector pSP64 Poly（A）. The recombinant vectors pET32 a（+）-AcTIP1-1 and pSP64 Poly（A）-AcTIP1-1 were successfully constructed. The former lays a foundation for inducing expression and purifying fusion protein to prepare antibodies and the latter for preparing AcTIP1-1 transcripts containing Poly（A）+.更多还原