【摘要】 利用微流控剪切力芯片系统为原代成骨细胞提供准确可控的流体剪切力(F_ss)刺激.实时监控成骨细胞钙信号在稳恒、脉冲流体作用下的变化,通过改变刺激强度、刺激时间、重复次数来研究成骨细胞对外部力学条件的准确感知和响应.实验结果表明:稳恒剪切力诱导的胞内钙响应曲线明显包含两个区间:钙峰区和平台区.钙峰区对力学强度敏感,而平台区对刺激时间敏感.在多次脉冲流体刺激下,钙响应随着重复次数逐步衰减,其衰减速度与刺激间隔时间成反比.更多还原

【Abstract】 We investigated the intercellular calcium([Ca²⁺]_i) responses in rat osteoblasts resulted from both steady and pulsed fluidic shear stress(F_ss) based on our design microfluidic device, by altering F_ss intensity, Fssstimulation time（width） and interval time. Results showed that steady F_ss obviously induced[Ca²⁺]_iresponses with two distinct phases: a peak phase and a plateau phase. The peak phase was sensitive to the F_ss intensity, while plateau phase was dependent on application of F_ss width. Meanwhile, a reductive tendency of [Ca²⁺]_iresponses was observed as experienced by different repetitive pulsed F_ss. This reductive tendency of [Ca²⁺]_iwas alleviated with increment of interval time.更多还原