【摘要】 [目的]本文旨在为评估复合性状转cry2A~*/bar基因水稻T2A-1自身杂草化的风险。[方法]在农田生态环境下,以T2A-1、受体水稻‘明恢63’(MH63)、南京本地常规籼型水稻‘中莲恢510’(LC)和江苏泰州杂草稻(WRTZ)为试验材料,测定供试材料在单种或混种条件下的营养和生殖生长指标,结合落粒性和种子生存能力,评价T2A-1的生存竞争能力。[结果]T2A-1、MH63、LC和WRTZ出苗率为92.62%～98.35%。T2A-1与MH63的抽穗期相同,为9月12日,但晚于LC 2 d,晚于WRTZ 5 d。在单种条件下,T2A-1的相对竞争能力与MH63及LC相当,显著高于WRTZ。在混种条件下,T2A-1的相对竞争能力与MH63相当,显著高于LC和WRTZ。T2A-1的落粒率(4.17%)与MH63(3.69%)相似,两者均显著低于LC(9.50%)和WRTZ(11.29%)。T2A-1的种子生存能力与MH63相似,均显著高于LC,显著低于WRTZ。[结论]在本试验条件下,T2A-1的竞争能力与受体水稻相当,但高于当地常规水稻,结合落粒性低和种子生存能力弱来综合分析,T2A-1的自身杂草化的风险较低。更多还原

【Abstract】 [Objectives]The paper aimed assessing the risk of weediness of stacked transgenic rice T2 A-1 with cry2 A~*/bar. [Methods]In this experiment,T2 A-1,its receptor rice‘Minghui 63’(MH63),Nanjing local conventional rice‘Zhonglianhui 510’(LC)and Jiangsu Taizhou weedy rice(WRTZ)were used as experimental materials. The survival competitive ability of T2 A-1,compared with MH63,LC and WRTZ,was evaluated by measuring the vegetative and reproductive variables,seed shattering and seed viability in pure planting or mixed planting with non-transgenic materials under field conditions. [Results]The emergence rate of T2 A-1,MH63,LC and WRTZ ranged from 92.62% to 98.35%. The heading date of T2 A-1 and MH63 was the same on September 12,which was 2 days later than LC,and 5 days later than WRTZ. Under the pure planting,the relative competitive ability of T2 A-1 was similar to those of MH63 and LC,but significantly greater than that of WRTZ. Under the mixed planting,the relative competitive ability of T2 A-1 was similar to that of MH63,but significantly greater than those of LC and WRTZ. The seed shattering of T2 A-1(4.17%)was similar to that of MH63(3.69%),and both of them were significantly lower than those of LC(9.50%)and WRTZ(11.29%). The seed viability of T2 A-1 was similar to that of MH63,significantly higher and lower than those of LC and WRTZ,respectively. [Conclusions]T2 A-1 had strong competitive advantages under the experimental conditions,and the potential weediness risk of T2 A-1 was very low according to comprehensive analysis of seed shattering,seed viability.更多还原