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杜鹃组培微枝试管外生根影响因素研究
Affecting Factors of Micro-cuttings Ex-vitro Rooting of Rhododendron
【摘要】 对杜鹃组培微枝试管外生根影响因素研究的结果表明,基质类型、基质处理方法、基质含水率、生长调节剂均对生根率有很大影响。在杜鹃无菌茎芽增壮培养基中添加0.8μmol/L的2-ip培养7~8周,用300 mg/L的GGR速蘸茎芽基部,扦插在均匀拌入菌虫双杀0.84 g/L的纯草炭土中(pH值5.8,含水率75%),在室内组培驯化室进行试管外生根可得到较好的生根效果。
【Abstract】 The results of research on influencing factors of Micro-cuttings Ex-vitro rooting of Rhododendron tissue culture showed that substrate type, substrate treatment method, substrate moisture content, and growth regulators all have a great effect on rooting rate. Add 0.8 mol/L of 2-ip to the sterile growth of rhododendron culture for 7~8 weeks. Quickly dip the base of the stem bud with 300 mg/L GGR,and cut into 0.84 g/L of double-killed bacteria pure peat soil(pH value of 5.8 and water content of 75%))can be better rooted when cultivated in an indoor tissue culture and domestication room for external rooting.
【Key words】 Rhododendron; Tissue culture; Micro-cuttings; Ex-vitro Rooting;
- 【文献出处】 林业科技 ,Forestry Science & Technology , 编辑部邮箱 ,2020年02期
- 【分类号】S685.21
- 【被引频次】1
- 【下载频次】232