【摘要】 目的:使用大蜡螟模型评价球形孢子丝菌伊曲康唑耐药株的毒力和对伊曲康唑的敏感性。方法:使用球形孢子丝菌伊曲康唑耐药株和敏感株分别建立大蜡螟幼虫感染模型,观察大蜡螟幼虫的外观变化、身体反馈及存活情况。向感染球形孢子丝菌的大蜡螟幼虫体内分别以20 mg/kg和40 mg/kg的剂量注射伊曲康唑,检测大蜡螟幼虫的存活情况和载菌量。结果:观察终点时,大蜡螟幼虫耐药株组和敏感株组之间的生存曲线无统计学差异(χ~2=2.37,P=0.124);注射40 mg/kg的伊曲康唑后,感染球形孢子丝菌耐药株和敏感株的大蜡螟生存率分别提高40%和60%,两者之间无统计学差异(χ~2=1.20,P=0.466),敏感株感染的大蜡螟菌落计数降低更为明显(t=-18.64,P<0.001)。结论:在大蜡螟模型中,球形孢子丝菌伊曲康唑耐药株的毒力和敏感株无差异,耐药株对伊曲康唑的敏感性低于敏感株。更多还原

【Abstract】 Objective:To evaluate virulence of itraconazole-resistant Sporothrix globosa and its sensitivity to itraconazole in Galleria mellonella. Methods:Itraconazole-resistant strain and itraconazole-sensitive strain of S. globosa were used to infect the Galleria mellonella larvae. Change in appearance, body feedback and survival of the larva were observed. Itraconazole was injected into the infected larvae at a dose of 20 mg/kg and 40 mg/kg, respectively. The survival and fungal burden of the larva were observed. Results: At the end of observation, there was no statistical difference in the survival curve between the resistant-strain group and sensitive-strain group(χ~2=2.37, P=0.124). After injecting itraconazole at 40 mg/kg, the survival rates of itraconazole-resistant strain and itraconazole-sensitive strain increased by 40% and 60%, respectively, and there was no statistical difference between them(χ~2=1.20, P=0.466).The colony count of CBS120340 was significantly lower than that of SUMS0381(t=-18.64,P<0.001). Conclusion: In Galleria mellonella, there was no difference in virulence between itraconazole-resistant strain and itraconazole-sensitive strain of S. globosa, and itraconazole-resistant strain showed lower sensitivity to itraconazole.更多还原