【摘要】 目的:初步预测及分析申克孢子丝菌(Sporothrix schenckii,Ss.)对羟苯基丙酮酸双加氧酶基因(4Hppd)编码蛋白的结构和生物学功能特征。方法:应用生物信息学在线工具预测分析Ss. 4Hppd编码蛋白的理化性质及功能特征。结果:Ss. 4Hppd基因编码蛋白含对羟苯基丙酮酸双加氧酶保守域,由477个氨基酸构成,理论分子量为52. 0 k D,等电点为5. 98,半衰期较长,该蛋白为非分泌蛋白,定位于线粒体中。其二级结构以无规则卷曲及α-螺旋为主,另外还存在乙二醛酶功能域及丰富的磷酸化位点,与其他致病真菌同源蛋白有较高的相似度。结论:预测Ss. 4HppD位于线粒体中,具有乙二醛酶功能域结构功能特点及丰富修饰位点,可为其表达、晶体结构分析及小分子抑制剂开发提供参考。更多还原

【Abstract】 Objective: To predict biological characterisics of 4-HppD,encoded by 4 Hppd in Sporothrix schenckii. Methods: Mutiple bioinformatics online tools were used to analyse the sequence. Results: Ss. 4 HppD,located in mitochodria,was formed by 477 amino acids and its theoretical molecular weight is 52. 0 k D. The isoelectric point was about 5. 98,with a long half life.The dominant secondary structures in Ss. 4 HppD were random coil and alpha helix. Besides,Ss.4 HppD had a similar tertiary structure with its homologue proteins,a glyoxalase motif at the carboxy end and multiple phosphorylation sites. Conclusion: Ss. 4 HppD was predicted to located in mitochondria,with a glyoxalase motif and various modification sites,which would facilitate the further researches in the protein including protein expression,function mechanism and inhibitior development.更多还原