【摘要】 目的检测微小RNA-193a-3p(miR-193a-3p)、生长基因1抑制剂(ING1)在食管癌组织中的表达水平,探讨两者的相关性及其与临床病理参数和预后的关系。方法 2013年1月～2014年6月于我院诊治的食管癌病人食管癌组织以及配对的癌旁组织标本90例,应用实时荧光定量PCR(qRT-PCR)检测食管癌和癌旁组织中miR-193a-3p的表达情况;免疫组化(IHC)检测食管癌和癌旁组织中ING1的表达情况;Target Scan7.1预测分析miR-193a-3p与ING1的靶向关系;采用Spearman秩相关分析检测miR-193a-3p与ING1在食管癌组织中表达的相关性;并分析miR-193a-3p、ING1的表达与食管癌病人临床病理参数及预后的关系。结果 miR-193a-3p在食管癌组织中的表达水平高于癌旁组织(P<0.05),ING1在食管癌组织中的阳性表达率低于癌旁组织(P<0.05)。Target Scan7.1预测ING1的3’UTR区存在miR-193a-3p的结合位点;食管癌组织中miR-193a-3p与ING1的表达呈负相关(P<0.05)。食管癌组织中miR-193a-3p、ING1的表达与淋巴结转移和TNM分期有关(P<0.05)。Kaplan-Meier生存曲线显示,miR-193a-3p高表达组食管癌病人生存率低于miR-193a-3p低表达组食管癌病人(P<0.05),ING1阴性表达的食管癌病人生存率低于ING1阳性表达组食管癌病人(P<0.05)。结论 miR-193a-3p、ING1在食管癌组织中均呈异常表达,且二者表达与食管癌病人的不良病理参数和预后相关,miR-193a-3p可能通过调控ING1促进食管癌的进展。更多还原

【Abstract】 Objective To detect the expression levels of microRNA-193 a-3 p(miR-193 a-3 p) and inhibitor of growth gene 1(ING1) in esophageal cancer tissue,and to explore the relationship between them and clinicopathological parameters and prognosis.Methods The esophageal cancer and adjacent paracancerous tissues were collected from 90 patients with esophageal cancer who were treated in our hospital from January 2013 to June 2014.Quantitative real-time PCR(qRT-PCR) was used to detect miR-193 a-3 p expression in esophageal cancer and adjacent paracancerous tissues.Immunohistochemistry(IHC) was used to detect ING1 expression in esophageal cancer and adjacent paracancerous tissues.Target Scan7.1 was used to predict and analyze targeting relationship of miR-193 a-3 p and ING1.Spearman rank correlation analysis was used to detecte the correlation between miR-193 a-3 p and ING1 expression in esophageal cancer tissues.The relationship between the expression of miR-193 a-3 p,ING1 and the clinicopathological parameters and prognosis of esophageal cancer were analyzed.Results The expression of miR-193 a-3 p in esophageal cancer tissues was significantly higher than that in adjacent paracancerous tissues(P<0.05),and the positive expression rate of ING1 in esophageal cancer tissues was lower than that in adjacent paracancerous tissues(P<0.05).Target scan 7.1 predicted that there was a binding site of miR-193 a-3 p in 3’UTR region of ING1.There was a negative correlation between the expression of miR-193 a-3 p and ING1 in esophageal cancer tissues(P<0.05).The expression of miR-193 a-3 p and ING1 in esophageal cancer was related to lymph node metastasis and TNM stage(P<0.05).Kaplan Meier survival curve showed that the survival rate of esophageal cancer patients with high expression of miR-193 a-3 p was lower than that of esophageal cancer patients with low expression of miR-193 a-3 p(P<0.05),and the survival rate of esophageal cancer patients with negative expression of ING1 was lower than that of esophageal cancer patients with positive expression of ING1(P<0.05).Conclusion The expressions of miR-193 a-3 p and ING1 are abnormal in esophageal cancer,and the expressions of miR-193 a-3 p and ING1 are related to the poor pathological parameters and prognosis of esophageal cancer.Mir-193 a-3 p may promote the development of esophageal cancer by regulating ING1.更多还原