【摘要】 目的:探讨咀嚼对应激大鼠杏仁核、前额叶皮质GABA再摄取的影响。方法:将成年雄性SD大鼠52只分成对照组(CO组)、应激组(ST组)、应激+咀嚼组(SC组)。除CO组外,各组大鼠均置于固定筒中束缚30 min。造模同时,SC组给予直径5 mm木棍以供其咀嚼,咀嚼少于15 min者被丢弃。通过旷场实验记录各组大鼠运动得分以测试其焦虑程度,实验结束后随机挑选大鼠18只(每组6只)立即处死,取杏仁核、前额叶皮质组织检测γ-氨基丁酸转运体-1(GAT-1)mRNA表达情况。结果:与CO组相比,ST组大鼠OF值显著降低,杏仁核与前额叶皮质GAT-1 mRNA表达显著升高(P<0.05);SC组大鼠OF值较ST组显著升高,杏仁核与前额叶皮质GAT-1 mRNA表达显著降低(P<0.05),但与CO组比较差异无统计学意义(P>0.05)。结论:咀嚼可以通过纠正杏仁核、前额叶皮质GAT-1的异常表达调节GABA代谢失常而发挥缓解压力的作用。更多还原

【Abstract】 Objective: To investigate the effect of mastication on GABA reuptake in amygdala and frontal cortex of rats. Methods: 52 SD rats were divided into control group(CO group), stress group(ST group), and stress + chewing group(SC group). Except for CO group, rats in each group were placed in a fixed tube for 30 minutes. Meanwhile, the SC group was given a 5 mm diameter stick for chewing, and those chewing for less than 15 minutes were discarded. The motor scores of each group were recorded in Open-Field test. At the end of the experiment, 18 rats(6 in each group) were randomly selected and killed immediately. Results: Compared with CO group, the OF value of ST group was significantly lower, and the expression of GAT-1 mRNA in amygdala and frontal cortex was significantly higher(P<0.05). The OF value of SC group was significantly higher than ST group, and the expression of GAT-1 mRNA in amygdala and frontal cortex was significantly lower(P<0.05). There was no significant difference between SC group and CO group(P>0.05). Conclusion: Mastication can relieve the stress by correcting the abnormal expression of GAT-1 in amygdala and frontal cortex.更多还原