【摘要】 目的:舌鳞状细胞癌(TSCC)是头颈部最常见的恶性肿瘤之一,其分子机制尚不清楚。挖掘与舌鳞状细胞癌(TSCC)密切相关的hub基因,为揭示TSCC发病机制提供理论依据。方法:从基因表达综合数据库(GEO)下载GSE34105数据,通过WGCNA筛选关键模块,Cytoscape计算模块网络中节点的拓扑参数筛选hub基因,生存分析用于hub基因的外部验证,通过GO和KEGG富集分析和GSEA分析获取hub基因参与的生物过程。结果:获得与TSCC最密切相关的turquoise模块(Cor=0.88,P<0.05)。cytoscape筛选获得2个hub基因分别为CAP1和TMBIM6,是预后的不良因素,它们的高表达与患者生存期缩短显著相关(P<0.05)。GSEA分析它们高表达组主要参与了半胱氨酸和蛋氨酸代谢、霍乱弧菌感染、氧化磷酸化、DNA复制、基底切除修复、细胞减数分裂、蛋白质生成、果糖和甘露糖代谢、幽门螺杆菌感染中的上皮细胞信号转导。结论:本研究通过构建基因共表达网络筛选出2个hub基因,可能成为潜在的TSCC基因基因生物标志物,影响TSCC的发生及预后。更多还原

【Abstract】 Objective: To excavate hub genes closely related to tongue squamous cell carcinoma(TSCC). Methods: GSE34105 dataset was downloaded from Gene Expression Omnibus Database(GEO). Key modules were screened by weighted gene co-expression network analysis(WGCNA). Hub genes were screened by topological parameters of nodes in module networks, and survival analysis was used for external verification of hub genes. GO and KEGG enriched analysis and GSEA analysis were performed to obtain the biological processes involved in hub genes. Results: The turquoise module(Cor=0.88, P<0.05) which was most closely related to TSCC was obtained. Two hub genes, CAP1 and TMBIM6, were obtained based on Cytoscape, which were adverse prognostic factors. Their high expression was significantly associated with shortening the survival time of patients(P<0.05). GSEA analysis of their high expression group was mainly involved in cysteine and methionine metabolism, Vibrio cholerae infection, oxidative phosphorylation, DNA replication, basal excision repair, cell meiosis, protein production, fructose and mannose metabolism, Helicobacter pylori Epithelial signal transduction in infection. Conclusion: This study screened two hub genes by constructing a gene co-expression network, which may become a potential biomarker of TSCC gene, affecting the occurrence and prognosis of TSCC.更多还原