【摘要】 目的对云南省文山州2019年手足口病(hand,foot and mouth disease,hfmd)病原分离情况和14株柯萨奇病毒a16型(coxsackievirus a16,cv-a16)的基因特征进行分析。方法对文山州2019年hfmd实验室送到云南省疾病预防控制中心hfmd实验室的595份手足口病患者粪便标本进行病毒分离,并对分离到的74株肠道病毒(enterovirus,ev)vp1区基因进行扩增和测序定型,对cv-a16病毒进行基因特征和分子流行病学分析。结果共从595份标本中分离到74株ev,ev分离率为12.44%(74/595),其中a组病毒(enterovirus species a,ev-a)72株,占97.30%(72/74),ev-b组病毒2株,占2.70%(2/74),未分离到ev-c和ev-d组病毒。ev-a组病毒中,cv-a6病毒最多,共44株,占ev-a组分离数的61.11%(44/72),cv-a16次之,共14株,占ev-a组病毒的19.44%(14/72),ev-a10 9株,占12.50%(9/72)。基因进化分析表明,14株cv-a16均为b1基因亚型(subgenotype b1)。它们分为2个不同的进化分支(b1a和b1b),其中8株为b1a,6株为b1b。结论 2019年文山州hfmd病原以cv-a6组为主,cv-a16次之。cv-a16病毒b1a和b1b两个分支同时在文山州流行。更多还原

【Abstract】 Objective To isolate the enterovirus(EV)from 595 stool samples collected from hand,foot and mouth disease( HFMD) patients from Wenshan prefecture of Yunnan province in 2019 and to analyze the epidemiology of EVs and the genetic characteristics of coxsackievirus A16(CV-A16). Methods Virus isolated from 595 stool samples was carried out and the VP1 gene of 74 positive samples was amplified and identified by sequencing and the genetic characteristics and molecular epidemiology of CV-A16 were analyzed. Results Seventyfour strains of EVs were isolated from 595 samples with an isolation rate of 12.44(74/595),of which,72 strains were enterovirus species A( EV-A), accounting for 97.30%( 72/74), 2 strains were EV-B, accounting for2.70%(2/74),EV-C and EV-D viruses were not isolated. Among EV-A viruses,CV-A6 was most frequently isolated,accounting for 61.11%(44/72),CV-A16 was the second,accounting for 19.44%(14/72),9 strains were CV-A10,accounting for 12.50%(9/72). The genetic characteristics analysis showed that all 14 strains of CV-A16 belonged to subgenotype B1,they were further divided into 2 subgenotypes(B1 a and B1 b),among which,8 strains were B1 a subgenotype,6 strains were B1 b subgenotype. Conclusions The main etiology of HFMD in Wenshan prefecture in 2019 was CV-A6,CV-A16 was the second. Two subgenotypes(B1 a and B1 b)of CV-A16 cocirculated in Wenshan prefecture in 2019.更多还原