【摘要】 目的:建立盐酸副玫瑰苯胺法和离子色谱法测定当归药材中二氧化硫残留量的方法,并比较2种测定方法对不确定度评估的影响。方法:建立测定当归药材中二氧化硫残留量的数学模型,分析影响其不确定度的因素来源,评定各个不确定度因素,并计算合成不确定度,最终得出当归药材中二氧化硫残留量结果在95%置信区间下(95%CI)的扩展不确定度;比较2种检测方法的不确定度、精密度、准确度、灵敏度、繁简性及可行性。结果:试验结果显示,采用盐酸副玫瑰苯胺法测定当归药材中二氧化硫残留量为(972.45±48.29)μg/g,最低检出限为2.79 mg/kg;而离子色谱法测定当归药材二氧化硫残留量为(1 170.53±40.5)μg/g,最低检出限为0.02 mg/kg;后者的可行性高于前者。结论:离子色谱法的检测灵敏、专属、准确、自动化程度高,可适用于中药材中残留二氧化硫的检测,其综合评价高于盐酸副玫瑰苯胺法。更多还原

【Abstract】 Objective: To establish a method for the determination of residual sulfur dioxide in Angelicae Sinensis Radix by pararosaniline hydrochloride(PRH) and ion chromatography(IC), and to compare the influence of the two methods on uncertainty assessment. Methods: A mathematical model was established for the determination of sulfur dioxide residues in Angelicae Sinensis Radix, the sources of factors affecting the uncertainty were analyzed, each uncertainty factor was evaluated, and the synthetic uncertainty was calculated. Finally, the extended uncertainty of the sulfur dioxide residues in Angelicae Sinensis Radix under the 95% confidence interval(95% CI) were derived. The uncertainty,precision, accuracy, sensitivity, complexity and feasibility of the two methods were compared. Results: The results showed that the residue of sulfur dioxide in angelica was(972.45±48.29) g/g by PRH method, and the minimum detection limit was 2.79 mg/kg.The residue of sulfur dioxide in angelica was(1 170.53±40.5) g/g by IC method, and the minimum detection limit was 0.02 mg/kg. The latter(by IC) is more feasible than the former(by PRH).更多还原