【摘要】 低碳氮比污水脱氮是城市污水处理的主要难题之一。自养脱氮技术由于不需要有机碳源和能耗低等优势,成为近年来污水处理研究的热点。本研究以EGSB反应器构建了自养脱氮工艺系统,解析了L-半胱氨酸对自养脱氮反应器的启动速度、脱氮效率和微生物群落结构等的影响。试验结果显示,以活性污泥作为接种物经过120d的启动过程,EGSB反应器的氨氮去除率达95.4%,亚硝酸盐去除率可达79.7%,总氮去除率可达72.7%。L-半胱氨酸的添加加快了反应器对高浓度N进水的适应速度,同时L-半胱氨酸有利于维持反应器处于较低溶解氧水平。Illumina Hiseq测序结果显示两个EGSB反应器中的主要优势菌属是不同类型的氮转化细菌,未添加L-半胱氨酸的反应器AnS1中以反硝化菌Rhodanobacter为主,而添加了L-半胱氨酸的反应器AnS2中以自养型菌属Nitrospira为主。本研究表明L-半胱氨酸有利于自养脱氮反应器的启动和氮转化微生物的富集。更多还原

【Abstract】 Nitrogen removal from wastewater with low C/N ratio is one of the main problems in municipal wastewater treatment.Autotrophic nitrogen removal technology has become a research hotspot in recent years due to its advantages of no organic carbon source and low energy consumption.The effects of L-cysteine on the start-up speed,nitrogen removal efficiency and microbial community structure of autotrophic denitrification reactor were analyzed.The experimental results showed that after 120 days of activation with activated sludge as the inoculum,the ammonia nitrogen removal rate of the EGSB reactor reaches 95.4%,the nitrite removal rate can reach 79.7%,and the total nitrogen removal rate can reach 72.7%.The addition of L-cysteine can accelerate the adaptation speed of the reactor to high-concentration N influent,and L-cysteine can keep a low concentration of oxygen in the reactor.Illumina Hiseq sequencing results showed that the main dominant bacteria in the two EGSB reactors were denitrification-related bacteria.The reactor AnS1 without L-cysteine was mainly denitrifying bacteria Rhodanobacter. The reactor AnS2 supplemented with L-cysteine is mainly autotrophic Nitrospira.This study showed that L-cysteine was beneficial to start-up of autotrophic denitrification reactor and the enrichment of nitrogen transforming microorganisms.更多还原