【摘要】 目的探讨miR-27a rs895819(T>C)的基因多态性与广东地区冠心病遗传易感性的关系,为冠心病的防治提供新思路。方法提取广东地区563例冠心病患者与650例排除冠心病的对照者血液基因组DNA,使用聚合酶链反应-连接酶检测反应(PCR-LDR)方法对标本进行基因分型。采用非条件Logistic回归评价该位点基因多态性与冠心病风险的相关性。结果 TT、CT、CC基因型在病例组中的分布频率分别为5.7%、38.0%、56.3%,在对照组中分别为5.9%、37.3%、56.8%。两组间基因型分布差异无统计学意义(P>0.05)。携带miR-27a rs895819T等位基因或C等位基因与冠心病的遗传易感性无明显相关性(OR=0.94,95%CI=0.72～1.27,P=0.518)。结论 miR-27a rs895819(T>C)的基因多态性与广东地区冠心病遗传易感性无相关性。更多还原

【Abstract】 Objective To explore the relationship between miR-27a rs895819(T>C)gene polymorphism and genetic susceptibility to coronary heart disease(CHD)in Guangdong area to provide a new idea for the prevention and treatment of CHD.Methods Blood genomic DNA in 563 cases of CHD and 650 controls excluding CHD in Guangdong area was extracted and conducted the genotyping by polymerase chain reaction-ligase detection reaction(PCR-LDR).The correlation between the miR-27a rs895819 gene polymorphism and the risk for CHD was evaluated by adopting the unconditional Logistic regression analysis.Results The distribution frequencies of TT,CT,and CC genotypes in the CHD case group were 5.7%,38.0% and 56.3% respectively,which in the control group were 5.9%,37.3% and 56.8% respectively,the difference in genotype frequency distribution between the two groups had no statistical significance(P>0.05).Carrying the miR-27a rs895819 Tallele or C allele had no significant correlation with genetic susceptibility to CHD(OR=0.94,95%CI=0.72-1.27,P=0.518).Conclusion miR-27a rs895819(T>C)gene polymorphism has no correlation with the susceptibility to CHD in the Guangdong area.更多还原