【摘要】 目的:探讨趋化因子CX3CL1对小鼠小胶质细胞铁吸收的影响。方法:用6-羟基多巴胺(6-OHDA)或柠檬酸铁铵(FAC)处理多巴胺能神经元细胞系MES23.5细胞,通过ELISA法检测MES23.5细胞趋化因子CX3CL1的释放;用CX3CL1和含CX3CL1的MES23.5条件培养基直接处理小鼠小胶质细胞系BV2细胞,或预先干扰CX3CL1受体CX3CR1后再行处理,采用钙黄绿素指示剂(Calcein-AM)观察BV2细胞对二价铁离子的吸收变化。结果:6-OHDA和FAC促进MES23.5细胞CX3CL1的释放,外源和内源性CX3CL1都可诱导BV2细胞对二价铁的吸收,这种诱导作用可被CX3CR1干扰阻断。结论:多巴胺能神经元释放的趋化因子CX3CL1可通过CX3CR1促进小胶质细胞对铁的吸收。更多还原

【Abstract】 Objective : To investigate the effect of fractalkine( CX3 CL1) on iron uptake of microglia in mice.Method: Dopaminergic MES23.5 cells were treated with 6-hydroxydopamine(6-OHDA) or ferric ammonium citrate(FAC)for 12 h. After another 24 h culture, the content of CX3 CL1 in supernatant medium of MES23.5 cells was measured by ELISA. The ferrous uptake of microglial BV2 cells was detected by using Calcein-AM indicator on divalent metal ions after they were treated directly with CX3 CL1 or conditioned medium containing CX3 CL1 from MES23.5 cells, and pre-treated with lentivirus encoded CX3 CR1 shRNA before then. Results: 6-OHDA and FAC promoted the release of CX3 CL1 from MES23.5 cells into medium. Both exogenous and endogenous CX3 CL1 induced the ferrous uptake of BV2 cells, but could be inhibited by CX3 CR1 shRNA. Conclusion: CX3 CL1 secreted from dopamin ergic neurons could facilitate the ferrous uptake of microglia, which was mediated by its receptor CX3 CR1.更多还原