【摘要】 目的探讨靶向肝癌磷脂酰肌醇蛋白聚糖（GPC）-3的纳米探针Ag₂S@BSA-TJ12P1在近红外二区（NIR-Ⅱ）成像早期诊断肝癌转移灶中的优势。方法制备硫化银纳米探针Ag₂S@BSA-TJ12P1。通过体外细胞实验验证GPC-3表达,检测各肝癌细胞系转移能力。采用激光共聚焦成像验证硫化银纳米探针选择性摄取至肝癌细胞系情况。采用肝癌高转移细胞系建立裸鼠肝癌原位转移模型,通过NIR-Ⅱ在体成像观察微小转移灶。结果 Ag₂S@BSA-TJ12P1被激发后,可在1 080 nm处发出NIR-Ⅱ荧光。细胞实验显示,肝癌GPC-3在肝癌细胞系中均有高表达,Ag₂S@BSA-TJ12P1可被HepG2、MHCC97-H、HCC-LM3细胞特异性摄取。NIR-Ⅱ成像系统对裸鼠微小转移灶作出早期诊断。结论 Ag₂S@BSA-TJ12P1可通过在体靶向肝癌GPC-3对肝癌微小转移灶成像,对肝癌微小转移灶早期诊断具有一定意义。更多还原

【Abstract】 Objective To investigate the advantages of the second near-infrared region(NIR-Ⅱnanoprobe（Ag₂S@BSA-TJ12P1） targeting liver cancer（Glypican-3, GPC-3） in the early diagnosis of liver cancer metastases by near-infrared second-region imaging. Methods Silver sulfide nanoprobe（Ag₂S@BSA-TJ12P1）was prepared. The expression of GPC-3 was verified by in vitro cell experiments, and the transferability of each cell line was tested. The selective uptake of silver sulfide nanoprobe into HCC cell lines was verified by laser confocal imaging. In situ metastasis of hepatocellular carcinoma in nude mice was established by using high metastatic cell lines of hepatocellular carcinoma. The micro-metastatic lesions were observed by NIR-Ⅱimaging. Results After Ag₂S@BSA-TJ12P1 was excited, it could emit near-infrared fluorescence at 1 080 nm.Cell experiments showed that GPC-3 was highly expressed in liver cancer cell lines, and Ag₂S@BSA-TJ12P1 could be specifically absorbed by HepG2, MHCC97-H and HCC-LM3. Early diagnosis of micro-metastatic nodules in nude mice could be made by NIR-Ⅱ imaging system. Conclusion The Ag₂S@BSA-TJ12P1 based on GPC-3 targeting liver cancer can visualize the micro-metastases of liver cancer by targeting the GPC-3 of liver cancer in vivo, which has certain potential for the early diagnosis of the micro-metastases of liver cancer.更多还原