【摘要】 目的以野生型和肝脏转基因T3斑马鱼为研究对象,探究硼砂对斑马鱼的发育毒性。方法不同浓度的硼砂分别处理斑马鱼胚胎,以受精后小时(hpf)为时间段,每24h观察并统计野生型斑马鱼的自主活动次数、孵化率、心率、畸形率、死亡率以及肝脏发育面积。结果斑马鱼胚胎24hpf,随硼砂浓度升高,硼砂各浓度处理组自主活动次数逐渐降低,0.6mg/ml硼砂组与空白对照组差异具有统计学差异(P <0.01)。与空白对照组相比,斑马鱼胚胎72hpf和96hpf,硼砂各浓度处理组斑马鱼心率显著降低(P <0.01); 96hpf,0.9mg/ml硼砂组的畸形率显著性升高(P <0.01); 96hpf和120hpf,0.9mg/ml硼砂组的死亡率显著性升高(P <0.01); 120hpf和144hpf,0.9mg/ml硼砂组斑马鱼幼鱼的肝脏面积显著减小(P <0.01)。结论高浓度的硼砂对斑马鱼具有发育毒性。更多还原

【Abstract】 Objective To study the developmental toxicity of borax to zebrafish of wild and liver transgenic T3( lfabp: EGFP). Methods Zebrafish embryo were treated with different concentrations of borax. The number of spontaneous movement,hatching rate,heart rate,malformation,mortality and liver area of zebrafish were observed and counted every 24 hours. Results When zebrafish eggs were fertilized for 24 hours,the number of spontaneous movement gradually was decreased with the concentrations of borax increased. There were statistical difference when the concentrations of borax reached 600 mg/L( P < 0. 01). When zebrafish eggs were fertilized for 72 and 96 hours,the heart rate in borax group significantly was decreased( P < 0. 01). When zebrafish eggs were fertilized for 96 hours,the malformation in borax group( 900 mg/L) was significantly increased( P < 0. 01). When zebrafish eggs were fertilized for 96 and 120 hours,the mortality in borax group( 900 mg/L) was sharply increased( P < 0. 01). When zebrafish eggs were fertilized for 120 and144 hours,the liver area of zebrafish juvenile in borax group( 900 mg/L) significantly decreased( P < 0. 01). Conclusion High concentrations of borax had developmental toxicity for zebrafish.更多还原