【摘要】 目的:构建小鼠股骨远端骨缺损植入模型.方法:8周龄雌性C57BL/6J小鼠股骨远端钻开1 mm直径的孔洞;将直径为1 mm、长度为5 mm的磺化聚醚醚酮植入小鼠股骨骨髓腔内;观察小鼠术后运动情况;术后2周,取材观察植入材料位置;使用micro-CT扫描三维重建小鼠骨微结构.结果:小鼠术后腿部行动自由,健康状况良好且存活至取材,取材后观察植入材料无松动脱出,股骨干完好未见缺损; microCT三维重建结果显示植入物位置正确.结论:使用该方法可成功构建小鼠股骨远端缺损模型.更多还原

【Abstract】 Objective: Establishing a mouse femur bone defection model. Methods: Three 8-week-old female C57 BL/6 J mouse were used in this experiment. A hole with 1 mm diameter was drilled on the distal femur.Then,the sulfonated polyetheretherketone( PEEK) with 1 mm diameter and 5 mm length was implanted into the femur marrow cavity. Micro-CT was used to reconstruct the bone microstructure and observe the implant position tow weeks later.Results: The health condition of mice with bone defection is good post-surgery and can survive until sacrifice.The movement of them is unlimited.The photos show that the implantation is fine placed in the femur marrow cavity.There is not any obvious damage.3 D reconstructed micro-CT images show that the position of implantation is fine. Conclusion: The mouse femur defection model was successfully established in this work.更多还原