【摘要】 目的利用敲除鼠探索锌指BED结构域超家族成员ZBED3在小鼠皮质发育过程中的作用。方法利用原位杂交和免疫荧光技术检测Zbed3敲除鼠的敲除效率。利用免疫荧光技术检测Zbed3敲除对小鼠皮质神经前体细胞增殖的影响。利用EdU标记技术和免疫荧光技术检测Zbed3敲除对皮质层次形成的影响。利用RT-qPCR和免疫印迹技术探索Zbed3敲除鼠中其他分子的表达变化。结果 Zbed3敲除鼠中检测不到Zbed3 mRNA和蛋白的表达。Zbed3敲除不影响神经祖细胞增殖和皮质层次形成。在Zbed3敲除鼠中,β-catenin的表达水平上调。结论 Zbed3敲除对小鼠皮质发育无明显影响。更多还原

【Abstract】 Objective To explore the role of zinc finger BED domain-containing protein 3, a member of zinc finger domain protein superfamily, in cortex cerebral development through conventional Zbed3 knockout mouse. MethodsIn situ hybridization and immunofluorescence were used to detect the mRNA and protein of Zbed3. Immunofluorescence was recruited to analyze the proliferation of neural progenitor cells in Zbed3 conventional knockout mouse. EdU labeling and immunofluorescence were used to analyze the neural position and layer fates in Zbed3 conven-tional knockout mouse. RT-qPCR and Western blot were used to detect the gene expression difference caused by Zbed3 knockout. Results Zbed3 mRNA and protein were not found in Zbed3 conventional knockout mouse; Knockout of Zbed3 failed to affect the proliferation of neural progenitor cells and the formation of neocortex layers. The expression of β-catenin was up-regulated in Zbed3 conventional knockout mouse. Conclusions Knockout of Zbed3 is not able to cause the apparent phenotype in cortex cerebral development.更多还原