【摘要】 目的分析探讨化学发光免疫分析法、酶联免疫吸附试验在乙型肝炎病毒标志物检验中的效果。方法方便选择该院2014年8月—2015年8月期间收治的200例乙型肝炎病毒患者为试验对象,所有患者入院之后需要采集静脉血,采集之后分别采取化学发光免疫分析法和酶联免疫吸附试验方法,每种方法检测人数为100例,分别检测乙型肝炎病毒标志物,对其检验结果进行分析比较。结果采用发光免疫分析法进行检测,其准确率达到96.00%,采用酶联免疫吸附实验方法进行检测,其准确率为84.00%,组间数据差异有统计学意义(χ~2=8.000,P<0.05),发光免疫分析法在HBsAg和HBeAg检测方面的阳性检测率为77.00%和35.00%,高于酶联免疫吸附实验方法检出率的60.00%和20.00%,其数据差异有统计学意义(χ~2=6.697、5.643,P<0.05)。结论对乙型肝炎病毒标志物进行检测,发光免疫分析法和酶联免疫吸附实验法的准确率均相对较高,但是发光免疫分析法更为显著。更多还原

【Abstract】 Objective To analyze and discuss the effects of chemiluminescence immunoassay and enzyme-linked immunosorbent assay in the detection of hepatitis B virus markers.Methods 200 cases of hepatitis B virus patients admitted to the hospital from August 2014 to August 2015 were convenient selected as experimental subjects.Venous blood was collected after all patients were admitted to hospital.Chemiluminescence immunoassay and enzyme-linked immunosorbent assay were adopted after collection.The number of people detected by each method was 100.Hepatitis B virus markers were detected respectively,and the test results were analyzed and compared.Results The accuracy of the method was96.00%.The accuracy of ELISA was 84.00%.There was statistically significant difference between the groups(χ~2=8.000,P<0.05).The positive detection rate of ELISA in HBsAg and HBeAg detection was 77.00% and 35.00%,which was higher than 60.00% and 20.0% of ELISA,the difference was statistically significant(χ~2=6.697,5.643,P<0.05).Conclusion For the detection of hepatitis B virus markers,the accuracy rate of luminescence immunoassay and enzyme-linked immunosorbent assay are relatively high,but luminescence immunoassay is more significant.更多还原