【摘要】 建立了高效液相色谱法同时测定色酚AS-PH反应液中色酚AS-PH、邻氨基苯乙醚和2-羟基-3-萘甲酸含量的方法。色谱柱为Inert Sustain C18（4.6mm×150mm,5μm）;检测波长为270nm;流动相为1g·L^-1磷酸二氢钾溶液（pH=3.0）-乙腈,梯度洗脱;流速为0.8mL·min^-1,外标法测定三种组分含量。结果表明:色酚AS-PH、邻氨基苯乙醚、2-羟基-3-萘甲酸与相邻色谱峰之间分离度良好,三种组分的浓度分别在2.0mg·L^-1～498mg·L^-1、2.0mg·L^-1～494mg·L^-1、1.0mg·L^-1～249mg·L^-1范围内线性关系良好,加标回收率分别为97.3%～103.7%、96.6%～102.0%、101.2%～104.6%。该方法操作简便、准确度高,可以为色酚AS-PH的工艺优化和中控分析提供依据。更多还原

【Abstract】 A high-performance liquid chromatography（HPLC）method was established for the determination of naphthol AS-PH,o-phenetidine and 2-hydroxy-3-naphthoic acid in naphthol AS-PH reaction solution.Chromatographic experiments were performed on Inert Sustain C18 column（4.6 mm×150 mm,5μm）,the mobile phase was 1 g·L^-1 potassium dihydrogen phosphate solution（pH=3.0）and acetonitrile in gradient elution,the flow rate was 0.8 mL·min^-1 and the detection wavelength was 270 nm,the contents were calculated by external reference method.The results showed that naphthol AS-PH,o-phenetidine and 2-hydroxy-3-naphthoic acid peaks were completely separated from adjacent peaks,and the three components had good linear relationships between 2.0 mg·L^-1～498 mg·L^-1、2.0 mg·L^-1～494 mg·L^-1、1.0 mg·L^-1～249 mg·L^-1 respectively,three levels standard were added to reaction solutions and the recoveries were 97.3%～103.7%、96.6%～102.0%、101.2%～104.6%respectively.The proposed method was simple and suitable for accurate determination of naphthol AS-PH,o-phenetidine and 2-hydroxy-3 naphthoic acid in naphthol AS-PH reaction solution,which would provide precise data for processing optimization and middle control analysis.更多还原