【摘要】 建立了河南刺梨中维生素C含量的反相高效液相测定方法,并测定了河南产刺梨中Vc的含量.色谱柱:Silgerrn TM C₁₈ （250 mm×4.6 mm,5μm）;柱温:室温;检测波长为245 nm;流动相为KH₂PO₄（0.05 mol/L）水溶液;进样量10μL;流速:1 mL/min;维生素C与相邻峰的分离度约为3;维生素C在8.16～14.28 mg/L范围内线性关系良好（r=0.992 5）,平均回收率为99.45%,RSD=1.6%（n=6）;该刺梨中维生素C的平均含量为2.103 mg/g.本方法操作简便,灵敏度高,分离度好,结果准确,重复性也比较好,适合河南产刺梨中维生素C的含量测定.更多还原

【Abstract】 A reversed-phase high-performance liquid chromatography determination method for vitamin C in Rosa roxburahii Tratt from Henan was established, and the content of vitamin C was determined. Separation was performed in TM C₁₈ column（250 mm×4.6 mm, 5 μm） at room temperature. The detection wavelength was set to 245 nm. KH₂PO₄（0.05 mol/L） solution was used as mobile phase with 1 mL/min. Injection volume was 10 μL. Resolution between vitamin C and adjacent peaks was about 3. Linear relationship was good（r=0.992 5） at range of 8.16-14.28 mg/L. Average recovery of vitamin C was 99.45% with RSD=1.6%（n=6）. The average content of vitamin C in the sample was 2.103 mg/g. This method was convenient, high sensitivity, accurate and suitable for the determination of vitamin C in Rosa roxburahii Tratt.更多还原