【摘要】 Au@Fe₃O₄作为固定化载体,以亲和素-生物素的特异性结合对氯过氧化物酶进行固定化,并对固定化酶的重复使用性进行了研究。结果表明,Au@Fe₃O₄载体是以Fe₃O₄为核Au为壳的核-壳型纳米材料,磁性分离效果较好,在酸性介质中较为稳定。固定化温度10℃、pH值为3.0、固定化时间40min,氯过氧化物酶的固载量相对较高。固定化酶在重复使用6次仍保留70%以上的酶残余活性,可以实现多次重复使用。更多还原

【Abstract】 Chloroperoxidase was immobilized with the carrier of Au@Fe₃O₄ by the method of specific binding of avidin-biotin, and the reusability of immobilized enzyme was studied. The results showed that the Au@Fe₃O₄ carrier was a core-shell nanoparticles with Fe₃O₄ as the core and Au as the shell. The magnetic separation effect of Au@Fe₃O₄ was good and stable in acid medium. With the temperature of 10℃, the pH 3.0, and the time of 40 min,the immobilized amount of chloroperoxidase was relatively high. After being reused for 6 times, the residual activity of immobilized enzyme was still more than 70%, which could be reused for many times.更多还原