【摘要】 目的:建立同时测定当归芍药散中芍药苷和芍药内酯苷含量的方法。方法:采用全波长HPLC,COSMOSIL 5C₁₈-MS-Ⅱ柱（4.6×250mm,5μm）,流动相:0.1%H₃PO₄溶液-乙腈（体积比86∶14）,检测波长为230nm,柱温为30℃,流速为1mL·min^-1。结果:芍药苷和芍药内酯苷分别在7.24～36.20μg·mL^-1,4.08～20.40μg·mL^-1线性关系良好,平均回收率分别为99.00%,96.80%。结论:该法简便、准确、灵敏、重现性好,可用于当归芍药散中芍药苷和芍药内酯苷的同时测定。更多还原

【Abstract】 OBJECTIVE To establish a method for determining peoniflorin and albiflorin in Danggui-shaoyao-sansimultaneously. METHOD A wavelength HPLC method was developed. The analysis was performed on a COSMOSIL 5C₁₈-MS-Ⅱ（4.6×250 mm, 5μm）Flow phase: 0.1% H₃PO₄-acetonitrile（volume ratio 86∶14）, The detection wavelengths were 230 nm, Column temperature is 30℃, The flow rate is 1 mL·min^-1. RESULTS The linear relationship is good for Paeoniflorin and Paeoniflorin between 7.24 ～36.20μg·mL^-1 and 4.08 ～20.40μg·mL^-1 respectively,with an average recovery rate of 99.00% and 96.80% respectively. CONCLUSION The method is simple, accurate,sensitive and reproducible, and can be used for determination of paeoniflorin and paeoniflorin in Danggui-shaoyao-san simultaneous.更多还原