【摘要】 目的:探讨三阴性乳腺癌患者PD-1/PD-L1通路表达改变及临床意义。方法:选取河北大学附属医院乳腺外科2016年1月～2019年10月收治的三阴乳腺癌患者50例作为研究对象,纳入研究组,选择同期于我院进行治疗的非三阴性乳腺癌患者55例作为对照,纳入对照组,采用RT-PCR法对PD-1、PD-L1在研究组和对照组、发生转移组与未转移组以及患者治疗前后的相对表达水平进行比较,采用Spearman相关性分析法对PD-1/PD-L1、PD-1/CD4、PD-L1/CD4进行相关性分析。结果:与对照组相比,三阴乳腺癌组患者癌组织及外周血中PD-1、PD-L1水平均明显升高;共有12例三阴乳腺癌患者发生肿瘤的局部转移,与未转移组相比,转移组三阴乳腺癌组患者癌组织及外周血中PD-1、PD-L1水平均明显升高;患者经过放化疗治疗后,与治疗前相比,患者PD-1、PD-L1;相关性分析结果表明,癌组织与外周血中PD-1/PD-L1水平均呈正相关关系,癌组织与外周血中PD-1、PD-L1水平与CD4+T淋巴细胞水平均呈负相关关系,均具有统计学意义。结论:三阴乳腺癌患者比普通乳腺癌患者癌组织及外周血中发生了明显的PD-1/PD-1通路上调,且与肿瘤转移、放化疗治疗存在联系,对于临床判别具有一定价值,其机制有待深入研究。更多还原

【Abstract】 Objective To investigate the change of PD-1 / PD-L1 pathway expression in triple negative breast cancer patients and its clinical significance.Methods 50 cases of triple negative breast cancer patients admitted to the Department of Breast Surgery of Hebei University Affiliated Hospital from January 2016 to October 2019 as the research object,and include them in the study group.Select 55 non-negative breast cancer patients treated in our hospital at the same time as Controls were included in the control group.The relative expression levels of PD-1 and PD-L1 in the study group and control group,the metastasis group and the non-metastatic group,and before and after treatment were compared by RT-PCR.Spearman correlation analysis was used.Correlation analysis was performed on PD-1/PD-L1,PD-1 / CD4,and PD-L1/CD4.Results Compared with the control group,the levels of PD-1 and PD-L1 in cancer tissues and peripheral blood of patients with triple-negative breast cancer were significantly increased,and the difference was statistically significant.There were 12 patients with triple-negative breast cancer.Local metastasis of the tumor occurred.Compared with the non-metastatic group,the levels of PD-1 and PD-L1 in the cancer tissue and peripheral blood of patients with metastatic breast cancer were significantly increased in the metastatic group,and the difference was statistically significant;After radiotherapy and chemotherapy,the patients’ PD-1 and PD-L1 levels were significantly lower than before treatment,and the difference was statistically significant;the correlation analysis showed that the cancer tissue and peripheral blood PD The level of PD-1/PD-L1 was positively correlated,and the levels of PD-1 and PD-L1 in cancer tissues and peripheral blood were negatively correlated with the levels of CD4+ T lymphocytes,which were statistically significant.Conclusion Patients with triple-negative breast cancer have significantly up-regulated PD-1 / PD-1 pathways in cancer tissues and peripheral blood compared with ordinary breast cancer patients,and are associated with tumor metastasis and chemoradiotherapy.It has certain value for clinical discrimination and its mechanism Need further research.更多还原