【摘要】 为从分子水平揭示甲基营养型芽胞杆菌WM7低温响应机制,采用高通量测序技术对低温和常温培养的菌体进行转录组测序分析,对部分差异表达显著基因进行RT-qPCR验证。结果表明,共检测到2 029个差异表达基因,其中1 519个低温上调,510个低温下调。GO和KEGG分析显示,1 199个差异表达基因归属细胞组分、分子功能和生物过程3个部分,683个基因参与到225个代谢途径中,其中涉及基因最多的是磷酸戊糖途径、嘌呤和氨基酸合成途径。菌株低温响应主要与脂肪酸代谢、ABC转运体、转录调控、信号转导、碳氮代谢等途径相关。此外,检测到大量差异表达的未知功能基因,多个基因差异表达倍数均大于50倍。本研究从基因表达方面阐述了甲基营养型芽胞杆菌WM7低温响应机制,为全面揭示其低温生长机制奠定了理论基础,提供了基因信息。更多还原

【Abstract】 To investigate the molecular mechanism of Bacillus methylotrophicus WM7 respond to low temperature stress, gene transcription profiles of strain WM7 grown at 30℃ and 15℃ were compared by transcriptome analysis. Some differentially expressed genes were verified by RT-qPCR. The results showed that a total of 2029 differentially expressed genes were found, of which 1519 were up-regulated and 510 were down-regulated. GO and KEGG analysis revealed that 1199 genes were categorized into cell component, molecular functions and biological process function groups and 683 genes were annotated in 225 metabolism pathways. These genes were significantly enriched in pentose phosphate pathway, purine and amino acid synthesis pathway. The significantly up-regulated genes at 15℃ were related to fatty acid metabolism, ABC transporters, transcriptional regulators, signal transduction, carbon and nitrogen metabolism, etc. In addition, a large number of differential expressed genes of unknown function were identified, and some of these genes were showed to be over 50-fold up-or down-regulated expression. This study facilitate to elucidate the molecular mechanism of strain WM7 respond to low temperature by gene expression research, which laid the foundation and provide the gene information for fully revealing the low temperature growth mechanism of strain WM7.更多还原