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靶向多肽功能化阳离子聚合物携载ZNF580质粒对内皮细胞增殖的影响

The Effect of Targeted Polypeptide Functionalized Cationic Polymer Loaded with ZNF580 Plasmid on Proliferation of Endothelial Cells

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【作者】 朵兴红姜萧韩周若琦冯亚凯刘晶

【Author】 DUO Xinghong;JIANG Xiaohan;ZHOU Ruoqi;FENG Yakai;LIU Jing;School of Chemistry and Chemical Engineering,Qinghai University for Nationalities;Key Laboratory of Resource Chemistry and Eco-environmental Protection in Tibetan Plateau,State Ethnic Affairs Commission;School of Chemical Engineering and Technology,Tianjin University;College of Eco-environment and Resources,Qinghai University for Nationalities;

【通讯作者】 朵兴红;

【机构】 青海民族大学化学化工学院青藏高原资源化学与生态环境保护国家民族事务委员会重点实验室天津大学化工学院青海民族大学生态环境与资源学院

【摘要】 通过原子转移自由基聚合(ATRP)方法制备了以多面体低聚倍半硅氧烷(POSS)为骨架的POSS-(PDMAEMA)8(PPD)星型阳离子聚合物,通过巯基-双键化学反应,将特异性靶向EA.hy926内皮细胞的CREDVW多肽键接在PPD阳离子聚合物末端,得到POSS-(PDMAEMA)8-PPEGMA-CREDVW(PPD-CREDVW)阳离子聚合物.在n(N)/n(P)=5时,PPD-CREDVW阳离子聚合物携载pEGFP-ZNF580(pDNA)质粒自组装形成粒径为125.67±3.31nm、zeta电位为10.64±1.65 mV的PPD-CREDVW/pDNA基因复合胶束.细胞实验结果显示:与PPD/pDNA基因复合胶束相比,PPD-CREDVW/pDNA基因复合胶束携载基因能力强、细胞毒性低、易被细胞摄取,能够显著提高EA.hy926内皮细胞的转染,促进细胞的增殖.

【Abstract】 A star-shaped cationic polymer POSS-(PDMAEMA)8(PPD) based on oligomeric sesquioxane(POSS)was prepared with atom transfer radical polymerization(ATRP). In order to prepare POSS-(PDMAEMA)8-PPEGMA-CREDVW(PPD-CREDVW) cationic polymer,the CREDVW peptide,which targets endothelial cells(EA.hy926),was grafted onto the end of PPD cationic polymer through the thiol-double bond click chemical reaction.When n(N)/n(P) = 5,PPD-CREDVW cationic polymer loaded with pEGFP-ZNF580(pDNA) plasmid could form PPD-CREDVW/pDNA gene composite micelle with a particle size of 125.6±3.31 nm and a zeta potential of 10.64±1.65 mV through self-assembly. Compared with PPD/pDNA gene complexes micelles,PPD-CREDVW/pDNA gene complexes micelles could be uptaken by cells,had strong capacity for gene-carrying and had low cytotoxicity. Thus it can significantly promote the transfection and proliferation of endothelial cells.

【基金】 国家自然科学基金项目(51963018);青海省自然科学基金项目(2019-ZJ-7013);教育部“春晖计划”合作研究项目(Z2015049);青海民族大学高层次人才项目(2019XJG01);青海民族大学2020年度校级本硕博(学生)项目(2020XJXS12)
  • 【文献出处】 华南师范大学学报(自然科学版) ,Journal of South China Normal University(Natural Science Edition) , 编辑部邮箱 ,2020年03期
  • 【分类号】Q78
  • 【被引频次】1
  • 【下载频次】124
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