【摘要】 通过原子转移自由基聚合（ATRP）方法制备了以多面体低聚倍半硅氧烷（POSS）为骨架的POSS-（PDMAEMA）₈（PPD）星型阳离子聚合物,通过巯基-双键化学反应,将特异性靶向EA.hy926内皮细胞的CREDVW多肽键接在PPD阳离子聚合物末端,得到POSS-（PDMAEMA）₈-PPEGMA-CREDVW（PPD-CREDVW）阳离子聚合物.在n（N）/n（P）=5时,PPD-CREDVW阳离子聚合物携载pEGFP-ZNF580（pDNA）质粒自组装形成粒径为125.67±3.31nm、zeta电位为10.64±1.65 mV的PPD-CREDVW/pDNA基因复合胶束.细胞实验结果显示:与PPD/pDNA基因复合胶束相比,PPD-CREDVW/pDNA基因复合胶束携载基因能力强、细胞毒性低、易被细胞摄取,能够显著提高EA.hy926内皮细胞的转染,促进细胞的增殖.更多还原

【Abstract】 A star-shaped cationic polymer POSS-（PDMAEMA）₈（PPD） based on oligomeric sesquioxane（POSS）was prepared with atom transfer radical polymerization（ATRP）. In order to prepare POSS-（PDMAEMA）₈-PPEGMA-CREDVW（PPD-CREDVW） cationic polymer,the CREDVW peptide,which targets endothelial cells（EA.hy926）,was grafted onto the end of PPD cationic polymer through the thiol-double bond click chemical reaction.When n（N）/n（P） = 5,PPD-CREDVW cationic polymer loaded with pEGFP-ZNF580（pDNA） plasmid could form PPD-CREDVW/pDNA gene composite micelle with a particle size of 125.6±3.31 nm and a zeta potential of 10.64±1.65 mV through self-assembly. Compared with PPD/pDNA gene complexes micelles,PPD-CREDVW/pDNA gene complexes micelles could be uptaken by cells,had strong capacity for gene-carrying and had low cytotoxicity. Thus it can significantly promote the transfection and proliferation of endothelial cells.更多还原