【摘要】 为研究磺胺甲恶唑对斑马鱼胚胎/仔鱼的早期发育及抗氧化系统的影响,将受精2h的斑马鱼胚胎暴露在不同浓度的磺胺甲恶唑水溶液中,观察斑马鱼胚胎死亡率、孵化率、仔鱼心包囊肿率、心跳数、体长以及仔鱼内抗氧化酶活性、丙二醛（MDA）含量的变化。结果表明,磺胺甲恶唑对斑马鱼胚胎/仔鱼120h的急性毒性半数致死质量浓度（LC50）为1 250.5mg/L;斑马鱼胚胎孵化率随着暴露浓度的增加呈现明显的下降趋势,96h胚胎孵化率抑制的半数有效质量浓度（EC50）为1 299.7mg/L;当暴露质量浓度≥1 500mg/L时,斑马鱼胚胎患心包囊肿率明显升高,呈现剂量—效应关系;经磺胺甲恶唑暴露96h的斑马鱼仔鱼30s心跳数及120h斑马鱼仔鱼体长总体降低;10^-2mg/L磺胺甲恶唑染毒96h后,即观察到斑马鱼仔鱼细胞出现明显的氧化损伤,过氧化氢酶的酶活性受到显著抑制,超氧化物歧化酶和谷胱甘肽S-转移酶被显著诱导,MDA含量显著增加。研究表明,磺胺甲恶唑暴露对斑马鱼胚胎细胞产生了明显的损伤,进而可能影响斑马鱼仔鱼的发育。更多还原

【Abstract】 The purpose of this study was to investigate the effect of sulfamethoxazole on the early development and antioxidant system of zebrafish（Danio rerio）embryo/larva.Zebrafish embryos were exposed to different concentrations of sulfamethoxazole,and the mortality,hatching rate,pericardial cyst rate,heart rate and body length of zebrafish embryo/larva were recorded everyday,and the effect of sulfamethoxazole on antioxidant enzyme activities and MDA contents was also analyzed.The results showed that the 120 h-LC50 of sulfamethoxazole for zebrafish was1 250.5 mg/L.The hatching rates of zebrafish embryos significantly decreased with the increase of exposure concentration,and the 96 h-EC50 of embryos hatching inhibition was 1 299.7 mg/L.When the exposure concentration was higher than 1 500 mg/L,the pericardial cyst rates of zebrafish embryos significantly increased and showed obvious dose-response relationship.In addition,exposured to sulfamethoxazole decreased the 30 seconds heart rates of zebrafish larvae after 96 hours and body length of zebrafish larvae after 120 hours in general.After exposure to 10^-2mg/L of sulfamethoxazole for 96 hours,significant oxidative damage was observed in the larvae,the CAT activity was significantly inhibited,the SOD and GST activity was significantly induced,and the MDA content was significantly increased.The studies indicated that sulfamethoxazole exposure caused obvious damage to zebrafish embryonic cells and affected the development of zebrafish larvae.更多还原