【摘要】 目的利用兔颈椎骨性结构测量数据建立兔颈椎椎间植骨融合内固定动物模型,观察不同时间点椎间融合情况。方法选取雄性健康新西兰大白兔36只(2.0～2.5 kg),随机分为A、B、C、D 4组,每组9只。A组用于解剖测量兔颈椎椎体结构;B、C、D组造模行颈椎椎间植骨融合内固定,植骨材料选用兔自体髂骨。术后4、8、12周对实验动物或标本分别进行大体观察、X线检查、手触检查及微型计算机断层扫描(micro computed tomography,Micro-CT)。结果自颈2(C2)～颈6(C6)椎体长度、椎体下端矢状径、椎体下端斜径差异有统计学意义(P<0.01)。X线检查显示B、C、D组植骨充分,钢板螺钉置入情况良好,仅1例术后发生螺钉松动退钉。B、C、D组手触检查颈椎融合率分别为22.2%(2/9)、55.6%(5/9)、88.9%(8/9)。C组新生骨体积与植入材料总体积比值和X线评分高于B组,D组BV/TV和X线评分高于B组和C组,差异有统计学意义(P<0.01)。结论颈椎椎间植骨融合内固定模型建立方法简便,为颈椎椎间融合的基础研究提供了一种可靠的动物模型。更多还原

【Abstract】 Objective According to the measurement data of cervical vertebrae structure in rabbits, the animal model of cervical interbody fusion and internal fixation was established. Cervical interbody fusion was observed at different time points. Methods Thirty-six healthy male New Zealand white rabbits(2.0-2.5 kg) were selected and randomly divided into groups A, B, C and D, with 9 rabbits in each group. Group A was used to measure the structure of cervical vertebrae in rabbits. The models in group B, C, and D were treated with cervical intervertebral bone grafting fusion and internal fixation, and the bone graft material was rabbit iliac bone. General observation, X-ray examination, manual palpation testing, and micro-computed tomography(Micro-CT) were performed at 4, 8, and 12 weeks after the operation. Results There were significant differences in the length of the vertebral body, sagittal diameter of lower vertebral body, and oblique diameter of lower vertebral body from C2 to C6(P<0.01). X-ray examination showed that in group B, C, and D, bone grafting was sufficient, plate and screw placement was good, and screw loosening and screw withdrawal occurred in only 1 case after surgery. The results of manual palpation showed that the cervical fusion rates in group B, C, and D were 22.2%(2/9), 55.6%(5/9), and 88.9%(8/9), respectively. The ratio of the volume of new bone to the total volume of implant material and X-ray score in group C were higher than those in group B, and the BV/TV and X-ray scores in group D were higher than those in group B and C, with statistically significant differences(P<0.01). Conclusion The method of establishing the anterior cervical interbody fusion model in rabbits is simple, which can provide a reliable animal model for the study of cervical interbody fusion.更多还原