【摘要】 利用溶剂提取法对显脉金花茶(Camellia euphlebia Merr.ex Sealy)干燥叶乙酸乙酯部位提取分离,并测定其含量。结果表明,从显脉金花茶叶片中乙酸乙酯部位首次分离得到化合物山奈酚(kaempferol);建立了HPLC法对显脉金花茶叶中山奈酚的含量测定方法,山奈酚含量在0.10～0.40μg/mL范围内线性关系良好,平均回收率为99.84%(RSD=1.59%),山奈酚含量为0.318 2～0.364 0 mg/g。更多还原

【Abstract】 Solvent extraction method was used to extract and separate the ethyl acetate part of dried leaves of Camellia euphlebia Merr.ex Sealy,and determine its content. The results showed that the compound kaempferol was first isolated from the ethyl acetate part of Camellia euphlebia Merr.ex Sealy;the content determination method of kaempferol in Camellia euphlebia Merr.ex Sealy was established by HPLC. The kaempferol ranged from 0.10 to 0.40 μg/mL. The internal linear relationship was good,the average recovery rate was 99.84%(RSD=1.59%),and the measured content of kaempferol was 0.318 2～0.364 0 mg/g.更多还原