【摘要】 为获得北虫草(Cordyceps militaris)CNZ虫草酸最适的提取方法,比较了6种虫草酸提取方法,结果表明超声辅助醇提法虫草酸的得率最高,达113.31 mg/g,可作为初始提取方法。进一步通过单因素试验和正交试验优化提取次数、固液比、超声时间、乙醇浓度等条件,获得北虫草CNZ虫草酸提取条件为:乙醇浓度20%(体积分数),固液比1∶10(g∶mL),超声时间20 min,提取次数3次;在优化的提取条件下,北虫草CNZ虫草酸得率为161.06 mg/g,与初始提取条件相比提高了42.1%。更多还原

【Abstract】 In order to obtain the optimal cordycepic acid extraction method of Cordyceps militaris CNZ,six extraction methods were used to extract cordycepic acid of C. militaris CNZ,respectively,and the extraction yield of cordycepic acid with ultrasonicassociated ethanol extraction method was the maximum for 113.31 mg/g,so was selected as one suitably initial method. Subsequently,the extraction conditions,including extraction times,solid-liquid ratio,ultrasonic time and ethanol concentration,were further optimized by single factor experiments and orthogonal experiments. One optimized cordycepic acid extraction method of C. militaris CNZ was obtained as that:ethanol concentration was 20%(volume fraction),solid-liquid ratio was 1∶10(g∶mL),ultrasonic time was 20 min and extraction times was three. Using the optimized extraction method,the extraction yield of cordycepic acid of C. militaris CNZ was 161.06 mg/g,which was about 42.1% higher than that using the initial extraction method.更多还原