【摘要】 光温敏核不育水稻的育性光温敏感度对两系杂交水稻的繁殖与制种有重要影响。为了定位影响温敏核不育水稻育性转换的低温敏感度QTL,以冷繁结实率存在明显差异的2个温敏核不育水稻N38S和N727S为亲本构建的F₂群体为作图群体,利用人工气候箱的低温处理和SSR标记技术,结合Mapmaker 3.0及WinQTLCart 2.5软件分析,检测低温敏感度QTL位点。结果显示,共检测到3个低温敏感度QTL,其中QTL1位于水稻第1染色体的PSM12与RM583之间,QTL2和QTL3分别位于第4染色体的PSM194与RM273之间和RM273与PSM103之间,它们加性效应均为负值,对育性转换的贡献率分别为7%,16%,3%。基于分子标记的位置信息可知,3个QTL位点与已定位的光温敏核不育水稻育性相关基因不等位。综上,QTL1、QTL2和QTL3可能是新基因位点,N727S携带的等位基因起到提高繁种产量的作用,在利用分子标记辅助选择时,应选择N727S的标记类型。这3个低温敏感度QTLs的定位可能对于温敏核不育水稻进行易于转育繁种的分子标记辅助选择具有重要意义。更多还原

【Abstract】 The photoperiod and temperature sensitivity of fertility reversibility has an important effect on the seed propagation and hybrid seed production of photo-and thermo-sensitive genic male sterile（PTGMS） rice. The aim of this study was to map QTLs of low temperature sensitivity affecting the fertility reversibility in thermo-sensitive genic male sterile（TGMS） rice. N38 S and N727 S were two TGMS rice which were obvious difference in seed setting rate of cold-water irrigation propagation. Using the F₂ population of N38 S×N727 S treated with low temperature in the artificial climate chamber and SSR marker technology, combined with Mapmaker 3.0 and WinQTLCart 2.5 software analysis, the QTLs of low temperature sensitivity were detected. The results showed that three QTLs of low temperature sensitivity were detected. The QTL1 was mapped to the interval between PSM12 and RM583 on chromosome 1. The QTL1 and QTL2 were mapped to the interval between PSM194 and RM273 and between RM273 and PSM103 on chromosome 4, respectively.Their additive effect was all negative value. To fertility reversibility, the contribution rate of the three QTLs was 7%, 16% and 3% in turn. Location information based on SSR markers suggested that the three QTLs were not the same as other reported genes related to fertility in PTGMS rice. In conclusion, QTL1,QTL2 and QTL3 may be new gene loci. The allele from N727 S plays a role in increasing the yield of seed propagation. When the three QTLs are used as molecular marker assisted selection, the molecular marker type of N727 S should be selected. So the three QTLs of low temperature sensitivity mapped in this study might be of great significance for molecular marker assisted selection of TGMS rice with a stable sterility but easy to reverse to fertility in seed propagation.更多还原